LOCUS       sequence-90516-e         841 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: N1 Cloning Vector, Excitation: 507 / 583, Emission: 
            517 / 593.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 841)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: wtKikGR-N1
FEATURES             Location/Qualifiers
     source          1..841
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    18..98
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      100..109
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             106..792
                     /codon_start=1
                     /product="photoconvertible Kikume green-to-red fluorescent 
                     protein (Tsutsui et al., 2005)"
                     /label=KikGR1
                     /translation="MVSVITSEMKIELRMEGAVNGHKFVITGKGSGQPFEGIQNVDLTV
                     IEGGPLPFAFDILTTAFHYGNRVFVEYPEEIVDYFKQSFPEGYSWERSMSYEDGGICLA
                     TNNITMKKDGSNCFVNEIRFDGVNFPANGPVMQRKTVKWEPSTEKMYVRDGVLKGDVNM
                     ALLLQGGGHYRCDFRTTYKAKKVVQLPDYHFVDHQMEITSHDKDYNKVKLYEHAKAHSG
                     LPRLAK"
ORIGIN
        1 agtgaaccgt cagatccgct agcgctaccg gactcagatc tcgagctcaa gcttcgaatt
       61 ctgcagtcga cggtaccgcg ggcccgggat ccaccggtcg ccaccatggt gagtgtgatt
      121 acatcagaaa tgaagatcga gctgcgtatg gaaggcgctg taaacgggca caagttcgtg
      181 attacaggga aaggaagtgg ccagcctttc gagggaatac agaatgtgga cctgacagtc
      241 atagagggcg gacctcttcc ttttgctttc gatatcctga caacagcatt ccattacggc
      301 aaccgggtat ttgtcgaata cccagaagaa atagtagact acttcaagca gtcgtttcct
      361 gagggttatt cttgggaacg aagcatgagt tacgaagacg ggggaatttg cctcgccaca
      421 aacaatataa cgatgaagaa agacggcagc aactgttttg tcaatgaaat tcgatttgat
      481 ggtgtgaact ttcctgccaa tggtccagtt atgcagagga agaccgtcaa atgggagcca
      541 tccactgaga aaatgtatgt gcgtgatgga gtgctgaagg gtgatgtaaa catggctctg
      601 ttgcttcaag gaggtggcca ttaccgatgt gacttcagaa ctacttacaa agcaaagaag
      661 gttgtccagt tgccagacta tcacttcgtg gatcatcaaa tggagataac aagccatgac
      721 aaggattaca acaaggttaa gctgtatgag cacgctaaag ctcattccgg gctgccaagg
      781 ctggccaagt aagcggccgc gactctagat cataatcagc cataccacat ttgtagaggt
      841 t
//
