LOCUS       sequence-90518-e         864 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: N1 Cloning Vector, Excitation: 548 / 634, Emission: 
            565 / 662.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 864)
  AUTHORS   Subach OM, Entenberg D, Condeelis JS, Verkhusha VV
  TITLE     A FRET-facilitated photoswitching using an orange fluorescent 
            protein with the fast photoconversion kinetics.
  JOURNAL   J Am Chem Soc. 2012 Aug 17.
  PUBMED    22900938
REFERENCE   2  (bases 1 to 864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Am Chem 
            Soc. 2012 Aug 17."
COMMENT     Sequence Label: PS-mOrange2-N1
FEATURES             Location/Qualifiers
     source          1..864
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    17..97
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      99..108
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             105..812
                     /codon_start=1
                     /product="enhanced orange to far-red photoswitchable 
                     monomeric fluorescent protein (Subach et al., 2012)"
                     /label=PSmOrange2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGTVNGHEFEIEGEGEGHPYEG
                     FQTAKLKVTKGGPLPFAWDILSPLITYGSKAYVKHPADIPDYFKLSFPEGFKWERVMNY
                     EDGGVVTVTQDSSLQDGEFIYKVKMRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIRMRLKLKDGGHYTSEVKTTYKAKKSVLLPGAYIVGIKLDITSHNEDYTIVEQYERS
                     EARHSTGGMDELYK"
     primer_bind     712..735
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 gtgaaccgtc agatccgcta gcgctaccgg actcagatct cgagctcaag cttcgaattc
       61 tgcagtcgac ggtaccgcgg gcccgggatc caccggtcgc caccatggtg agcaagggcg
      121 aggagaataa tatggccatc attaaggagt tcatgcgctt caaggtgcac atggagggca
      181 ctgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccacccc tacgagggct
      241 ttcagaccgc taagctgaag gtgaccaagg gcggccccct gcccttcgcc tgggacatcc
      301 tgtcccctct catcacctac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg
      361 actacttcaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aactacgagg
      421 acggcggcgt ggtgaccgtg acccaggact cctctctgca ggacggcgag ttcatctaca
      481 aggtgaagat gcgcggcacc aacttcccct ccgacggccc cgtgatgcag aagaagacca
      541 tgggctggga ggcctcctcc gagcggatgt accccgagga cggcgccctg aagggcgaga
      601 tcaggatgag gctgaagctg aaggatggcg gccactacac ctccgaggtc aagactacct
      661 acaaggccaa gaagtccgtg ctgctgcccg gcgcctacat cgtcggcatc aagctggaca
      721 tcacctccca caacgaggac tacaccatcg tggaacagta cgaacgctcc gaggcccgcc
      781 actccaccgg cggcatggac gagctgtaca agtaagcggc cgcgactcta gatcataatc
      841 agccatacca catttgtaga ggtt
//
