LOCUS       sequence-90522-e         863 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: C1 Cloning Vector, Excitation: 580, Emission: 608.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 863)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: mKate2.5-C1
FEATURES             Location/Qualifiers
     source          1..863
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      33..42
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             39..734
                     /codon_start=1
                     /product="monomeric red fluorescent protein optimized for 
                     use as a fusion tag (Shemiakina et al., 2012)"
                     /label=FusionRed
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     VVEGGPLPFAFDILATSFMYGSKTFINHPQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPANGPVMQKKTLGWEASTETLYPADGGLEGACDMAL
                     KLVGGGHLICNLETTYRSKKPAKNLKMPGVYNVDRRLERIKEADNETYVEQHEVAVARY
                     STGGAGDGGK"
     misc_feature    747..812
                     /label=MCS
                     /note="multiple cloning site"
ORIGIN
        1 gtgaaccgtc agatccgcta gcgctaccgg tcgccaccat ggtgagcgag ctgattaagg
       61 agaacatgca catgaagctg tacatggagg gcaccgtgaa caaccaccac ttcaagtgca
      121 catccgaggg cgaaggcaag ccctacgagg gcacccagac catgagaatc aaggtcgtcg
      181 agggcggccc tctccccttc gccttcgaca tcctggctac cagcttcatg tacggcagca
      241 aaaccttcat caaccaccct cagggcatcc ccgacttctt taagcagtcc ttccctgagg
      301 gcttcacatg ggagagagtc accacatacg aagacggggg cgtgctgacc gctacccagg
      361 acaccagcct ccaggacggc tgcctcatct acaacgtcaa gattagaggg gtgaacttcc
      421 cagccaacgg ccctgtgatg cagaagaaaa cactcggctg ggaggcctcc accgagacgc
      481 tgtaccccgc tgacggcggc ctggaaggcg catgtgacat ggccctgaag ctcgtgggcg
      541 ggggccacct gatctgcaac ttggagacca catacagatc caagaaaccc gctaagaacc
      601 tcaagatgcc cggcgtctac aacgtggaca ggagactgga aagaatcaag gaggccgaca
      661 atgagaccta cgtcgagcag cacgaggtgg ctgtggccag atactctact ggtggcgctg
      721 gtgatggagg taaaggtgga ggaggttccg gactcagatc tcgagctcaa gcttcgaatt
      781 ctgcagtcga cggtaccgcg ggcccgggat ccaccggatc tagataactg atcataatca
      841 gccataccac atttgtagag gtt
//
