LOCUS       sequence-90536-e         810 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: N1 Cloning Vector, Excitation: 551, Emission: 565.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Sakaue-Sawano A, Kurokawa H, Morimura T, Hanyu A, Hama H, Osawa H, 
            Kashiwagi S, Fukami K, Miyata T, Miyoshi H, Imamura T, Ogawa M, 
            Masai H, Miyawaki A
  TITLE     Visualizing spatiotemporal dynamics of multicellular cell-cycle 
            progression.
  JOURNAL   Cell. 2008 Feb 8;132(3):487-98. doi: 10.1016/j.cell.2007.12.033.
  PUBMED    18267078
REFERENCE   2  (bases 1 to 810)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2007.12"; journalName: "Cell"; date: "2008-02-8- 8";
            volume: "132"; issue: "3"; pages: "487-98"
COMMENT     Sequence Label: mKO2-N1
FEATURES             Location/Qualifiers
     source          1..810
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    17..97
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      99..108
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             105..761
                     /codon_start=1
                     /product="monomeric Kusabira-Orange 2 fluorescent protein"
                     /label=mKO2
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS"
ORIGIN
        1 gtgaaccgtc agatccgcta gcgctaccgg actcagatct cgagctcaag cttcgaattc
       61 tgcagtcgac ggtaccgcgg gcccgggatc caccggtcgc caccatggtg agtgtgatta
      121 aaccagagat gaagatgagg tactacatgg acggctccgt caatgggcat gagttcacaa
      181 ttgaaggtga aggcacaggc agaccttacg agggacatca agagatgaca ctacgcgtca
      241 caatggccga gggcgggcca atgcctttcg cgtttgactt agtgtcacac gtgttctgtt
      301 acggccacag agtatttact aaatatccag aagagatacc agactatttc aaacaagcat
      361 ttcctgaagg cctgtcatgg gaaaggtcgt tggagttcga agatggtggg tccgcttcag
      421 tcagtgcgca tataagcctt agaggaaaca ccttctacca caaatccaaa tttactgggg
      481 ttaactttcc tgccgatggt cctatcatgc aaaaccaaag tgttgattgg gagccatcaa
      541 ccgagaaaat tactgccagc gacggagttc tgaagggtga tgttacgatg tacctaaaac
      601 ttgaaggagg cggcaatcac aaatgccaaa tgaagactac ttacaaggcg gcaaaagaga
      661 ttcttgaaat gccaggagac cattacatcg gccatcgcct cgtcaggaaa accgaaggca
      721 acattactga gcaggtagaa gatgcagtag ctcatagcta agcggccgcg actctagatc
      781 ataatcagcc ataccacatt tgtagaggtt
//
