LOCUS       sequence-90540-e         852 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: N1 Cloning Vector, Excitation: 580, Emission: 515.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: Kindling-N1
FEATURES             Location/Qualifiers
     source          1..852
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    17..97
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      99..108
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             105..803
                     /codon_start=1
                     /product="photoactivatable nonfluorescent protein that 
                     becomes red fluorescent upon exposure to green light"
                     /label=KFP-Red
                     /note="mammalian codon-optimized"
                     /translation="MASLLTETMPFKTTIEGTVNGHCFKCIGKGEGNPFEGTQEMKIEV
                     IEGGPLPFAFHILSTSCMYGSKTFIKYVSGIPDYFKQSFPEGFTWERTTTYEDGGFLTA
                     HQDTSLDGDCLVYKVKILGNNFPADGPVMQNKVGRWEPGTEIVYEVDGVLRGQSLMALK
                     CPGGRHLTCHLHTTYRSKKPASALKMPGFHFEDHRIEIMEEVEKGKCYKQYEAAVGRYC
                     DAAPSKLGHN"
ORIGIN
        1 gtgaaccgtc agatccgcta gcgctaccgg actcagatct cgagctcaag cttcgaattc
       61 tgcagtcgac ggtaccgcgg gcccgggatc caccggtcgc caccatggcc tccctgctga
      121 ccgagaccat gcccttcaag accaccatcg agggcaccgt gaacggccac tgcttcaagt
      181 gcatcggcaa gggcgagggc aaccccttcg agggcaccca ggagatgaag atcgaggtga
      241 tcgagggcgg ccccctgccc ttcgccttcc acatcctgtc cacctcctgc atgtacggct
      301 ccaagacctt catcaagtac gtgtccggca tccccgacta cttcaagcag tccttccccg
      361 agggcttcac ctgggagcgc accaccacct acgaggacgg cggcttcctg accgcccacc
      421 aggacacctc cctggacggc gactgcctgg tgtacaaggt gaagatcctg ggtaacaact
      481 tccccgccga cggccccgtg atgcagaaca aggtcggccg ctgggagccc ggaaccgaga
      541 tcgtgtacga ggtggacggc gtgctgcgcg gccagtccct gatggccctg aagtgccccg
      601 gcggccggca cctgacctgc cacctgcaca ccacctaccg ctccaagaag cccgcctccg
      661 ccctgaagat gcccggcttc cacttcgagg accaccgcat cgagatcatg gaggaggtgg
      721 agaagggcaa gtgctacaag cagtacgagg ccgccgtggg ccgctactgc gacgccgccc
      781 cctccaagct gggccacaac tgagcggccg cgactctaga tcataatcag ccataccaca
      841 tttgtagagg tt
//
