LOCUS       sequence-90541-e         867 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin, Excitation: 400 / 490, Emission: 468 / 511.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: PS-CFP2-Lifeact-7
FEATURES             Location/Qualifiers
     source          1..867
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      23..32
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             29..79
                     /codon_start=1
                     /product="peptide that binds filamentous actin (Riedl et 
                     al., 2008)"
                     /label=Lifeact
                     /note="first 17 amino acids of budding yeast Abp140"
                     /translation="MGVADLIKKFESISKEE"
     CDS             101..817
                     /codon_start=1
                     /product="photoswitchable cyan-to-green fluorescent 
                     protein"
                     /label=PS-CFP2
                     /note="mammalian codon-optimized"
                     /translation="MSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVATLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDDG
                     NYKTRAEVKFEGDTLVSRIELTGTDFKEDGNILGNKMEYNYNATNVYIVADKARNGIKV
                     NFKVRHNIKDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYLE
                     FVTAAAITHGMDELYK"
ORIGIN
        1 gtgaaccgtc agatccgcta gcgccaccat gggcgtggcc gacttgatca agaagttcga
       61 gtccatctcc aaggaggagg gggatccacc ggtcgccacc atgagcaagg gcgccgagct
      121 gttcaccggc atcgtgccca tcctgatcga gctgaatggc gatgtgaatg gccacaagtt
      181 cagcgtgagc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
      241 ctgcaccacc ggcaagctgc ctgtgccctg gcccaccctg gtggccaccc tgagctacgg
      301 cgtgcagtgc ttctcacgct accccgatca catgaagcag cacgacttct tcaagagcgc
      361 catgcctgag ggctacatcc aggagcgcac catcttcttc gaggatgacg gcaactacaa
      421 gacgcgcgcc gaggtgaagt tcgagggcga taccctggtg agtcgcatcg agctgaccgg
      481 cactgatttc aaggaggatg gcaacatcct gggcaataag atggagtaca actacaacgc
      541 caccaatgtg tacatcgtgg ccgacaaggc caggaatggc atcaaggtga acttcaaggt
      601 ccgccacaac atcaaggatg gcagcgtgca gctggccgac cactaccagc agaatacccc
      661 catcggcgat ggccctgtgc tgctgcccga taaccactac ctgtccaccc agagcgccct
      721 gtccaaggac cccaacgaga agcgcgatca catgatctac ctcgagttcg tgaccgccgc
      781 cgccatcacc cacggcatgg atgagctgta caagtaaagc ggccgcgact ctagatcata
      841 atcagccata ccacatttgt agaggtt
//
