LOCUS       sequence-9055-ex        6034 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6034)
  AUTHORS   Merritt C, Rasoloson D, Ko D, Seydoux G
  TITLE     3' UTRs are the primary regulators of gene expression in the C. 
            elegans germline.
  JOURNAL   Curr Biol. 2008 Oct 14;18(19):1476-82. doi: 
            10.1016/j.cub.2008.08.013. Epub 2008 Sep 25.
  PUBMED    18818082
REFERENCE   2  (bases 1 to 6034)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2008.08.013"; journalName: "Curr Biol"; date: 
            "2008-10-14- 14"; volume: "18"; issue: "19"; pages: "1476-82"
COMMENT     Sequence Label: pCM3.32
FEATURES             Location/Qualifiers
     source          1..6034
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(73..100)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(192..278)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    331..430
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(4210..4309)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(4421..4440)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             4432..5241
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(4499..4518)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      5384..5972
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5873..5892
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgctagcc aggaagagtt
       61 tgtagaaacg caaaaaggcc atccgtcagg atggccttct gcttagtttg atgcctggca
      121 gtttatggcg ggcgtcctgc ccgccaccct ccgggccgtt gcttcacaac gttcaaatcc
      181 gctcccggcg gatttgtcct actcaggaga gcgttcaccg acaaacaaca gataaaacga
      241 aaggcccagt cttccgactg agcctttcgt tttatttgat gcctggcagt tccctactct
      301 cgcgttaacg ctagcatgga tctcgggccc caaataatga ttttattttg actgatagtg
      361 acctgttcgt tgcaacaaat tgatgagcaa tgctttttta taatgccaag tttgtacaaa
      421 aaagcaggct ccatgtctgg acagcagttc cagcaacatt cacagcagca acaacaacgt
      481 gccccaaccg tctataataa taacaacaag aaaaacggcg gcagcgctgc aaactcaaac
      541 caaaacaaca acaaccccca tcatacctca acgactgatt cggtgccgaa ctcgactgcc
      601 aacgtgtcgt cagcaatttc gtttgagtat tcagagccgc caggaaacac cactaggaca
      661 acaccagatt ctgggctagg gactgacgac caaaagggca cacctcaaca gacacgtgct
      721 ccgccaggat ttgtaagtag gaaactattg aaaatcctat cgaaaaaaga taagtcagaa
      781 aaagaacact tttgaggtgc gcaaggtttt aagagaattg agagattttt caaatcagtg
      841 aaatatcaaa catgtaattt atctcattca ttttcagcat cgagcagctt atcatcggaa
      901 cagcggtcaa cagcaattct ctcaaatgcg cccacagccg ccacatactg gaccaccaaa
      961 tggtatgaca tattacggag gaaccgtcgg cgcgcccggc tatcagcaac aacaaccacc
     1021 accacaagaa ggatatgatc aacagcagca gtttggaggt ggatccgatg gtggacaata
     1081 ccaaagagga ccaccaggag gctatcgggt atggagaatt acgggctcct caattatttc
     1141 tagctcataa taatttttta atgagcgtta tgatttggaa attatatcta caggaaaata
     1201 atgagaagag ttgatagaca ttttgcaaat tctttatttt cagcagtatc aacaaatgga
     1261 aagcggtggt ccgccaagtg gatacgcgga tgacaacaac ggtggaaaca ataattttgt
     1321 cattcatggc cccaacgata atttcgatgg tcggccacaa tatagttggt gcaaacttac
     1381 tgtgttctgt tcaaaagcat aattttagat cctcaagcag ggccaccacc acaaaatcag
     1441 cagcaaatga tgaacagtgg caactatgaa attgatcagt accgtggaca aggacctcct
     1501 ccaggaaata cctatcaaca gcagggacat agtggacaag ctcaatatta tgctggacca
     1561 gtaatccagc aacggccacc accgccacac aatgctcaat cttatggaca gtataggggt
     1621 ccacaaggcc cgcaaggcca aattattcaa atgatgcctc acaatccaca gaatcaacag
     1681 cagcagcaac aacatccaat gctgtatcca atggtgtatg ttcctgtgcc agcaccacga
     1741 gtgttccggc aagagccagc tggtggagct agcagtttgg aaaaagacgg aggaaatgct
     1801 cgcaaaagta aacgaaacag tcaaaatgag gacgatggtc agcaacatca acatcagaat
     1861 gaacaacaac ctcaacaagg ctaccatcaa caggattttg tatcgaaccc actctggaat
     1921 cagatgcctg tcggttacgc tccaccagga aacttcacgc agcctcagca acaacatcaa
     1981 caaatggatc aacaacaagc cgtccaaatg caacatcaga tgcaggtaca actttaaaaa
     2041 aattagggga agattttata ttatattttg acaatttcac agagttacac tttttttaaa
     2101 aagaacaaac aacaattcct cagatgtttc agcaaatgca accatacgga atgccacaag
     2161 gaggcccacc accacctcat gctgctgctt ctatgatgaa tcatccccat cagcaaaatc
     2221 atcctcatat gaatgcacaa atgacccaac aacagcatcc aggacaatat ctaccgcaac
     2281 aatatatgca aatggttccg ccgcctcacc atcaaatgca acaaatgcaa cagcaaggta
     2341 gacgtccagg aggtgttgag ccgctcatgg gaggcccgcc gtatcaaatg agtggacaaa
     2401 ttaatcaaat gggaggacca atacgtggag gttttggaat gcaacagcaa cagccgatgg
     2461 gctacttcaa ttctggaaga ggtggaaatg ctggatcagg aagaggaaga ggtggttcac
     2521 gaggtcacgg ataccaacat tctcgtcaaa attctaatca aagctacaat tcttctcaaa
     2581 gttatccaca aaatcgagga aattttgatt catcaagtca atatcaatcc gacagtcaac
     2641 aagtggatga atcagttaca cagcttacca atagaattga acatcttcaa ccagttagct
     2701 cacgtgcagg atcaccaagt aaacagatcc ttcagttagt cgaatcgaaa gatgatcaaa
     2761 cttcgactga aaattctgaa tcaccaagtc ggtcaagcac tcctaaacat cggaagaaga
     2821 ccccaaagaa agattttaaa gttgatcaag aagcatctat cgaagcgaca aaaactgaaa
     2881 taagcgagga aaatccgaaa acggatgata ttcagtctaa agatgatgtt acttcgaaat
     2941 ctgaactaca ctgttatcga tgtgaacatt accagttgcc tgctgaagaa gtgtcttccc
     3001 ataagtaaga ttaaaaattc taatttataa aaattaaaaa tttaatttca gtattcgcaa
     3061 ggataatggt gatttgtggt gtgagcatat gaaaaagatc aaatgcgggc attgtgaggc
     3121 gactggagaa caaggtcatc accctctaat ttgtccaaaa aagaaagaag aagaacgtgt
     3181 ggcaaaatct cgagagtctt cgcagaagcc gatcgatccg caggaaataa gtgatgatca
     3241 ggatgatact gtaccggatg ttcccgatca aatagtcgaa caagacaatc agtcacataa
     3301 atctcgtcac aattcagaaa atcgcagtgg cggtagagga tacggtcaga actatagcca
     3361 tagttatggc ggaaattcta ctggtagcta tcgtggacga tcgtctggag gaagtggagg
     3421 cagtggacgt ggtggtggtc gtggaggtta ctcacgtgga catggtggag gatcaggagg
     3481 aggacaacgt ggaaactatc aggtttgtat tttaataaat tgaaaaaaaa aactaattcc
     3541 catcaagttt ccagaataaa cgtaagtaaa atcgttggat tttgattggc accgatgtcc
     3601 aacgacaaca aaaacttcct ccgattttca agaatctttc aaatatcccg ctcgcccaaa
     3661 caaaattcac ttcgatctct attcattcca tcccatgcta ataccaccat cccaccgaat
     3721 tccaactgcg aaagctttaa aagaaacccc ctcatgtcta tcccgatttg ttgttgaaac
     3781 gttttgttgt ttttcttttc ttttttgttg ttttttttgc taaagaattc ccccaatcct
     3841 acaaggattc cgtaactcga gttccatcct ttggtctgtt cgaattcaat tcgtcatccc
     3901 ttttctgtca tgattttgtt gaaaattgaa atttttggca gaaaatcctg attttttaaa
     3961 attgtgtcac cgttctttct ttcttatact atctcccttc tcccccactt tctaaagtat
     4021 ctctataatc ccgcccgttt tcctcttttt ctctatcccc tttctcttcc ccatcaaaca
     4081 atgcataata ctgatgatgt tggttcgaaa aacgatcttc aaatatttat gtttccgata
     4141 aatctcaaaa ttcaaacaga agtgtcccgt attgttgttg ttgatcaatt agagaaaaca
     4201 agaacatcta cccagctttc ttgtacaaag tgggcattat aagaaagcat tgcttatcaa
     4261 tttgttgcaa cgaacaggtc actatcagtc aaaataaaat cattatttgc catccagctg
     4321 cagctctggc ccgtgtctca aaatctctga tgttacattg cacaagataa aaatatatca
     4381 tcatgaacaa taaaactgtc tgcttacata aacagtaata caaggggtgt tatgagccat
     4441 attcaacggg aaacgtcgag gccgcgatta aattccaaca tggatgctga tttatatggg
     4501 tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg cttgtatggg
     4561 aagcccgatg cgccagagtt gtttctgaaa catggcaaag gtagcgttgc caatgatgtt
     4621 acagatgaga tggtcagact aaactggctg acggaattta tgcctcttcc gaccatcaag
     4681 cattttatcc gtactcctga tgatgcatgg ttactcacca ctgcgatccc cggaaaaaca
     4741 gcattccagg tattagaaga atatcctgat tcaggtgaaa atattgttga tgcgctggca
     4801 gtgttcctgc gccggttgca ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc
     4861 gtatttcgtc tcgctcaggc gcaatcacga atgaataacg gtttggttga tgcgagtgat
     4921 tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat gcataaactt
     4981 ttgccattct caccggattc agtcgtcact catggtgatt tctcacttga taaccttatt
     5041 tttgacgagg ggaaattaat aggttgtatt gatgttggac gagtcggaat cgcagaccga
     5101 taccaggatc ttgccatcct atggaactgc ctcggtgagt tttctccttc attacagaaa
     5161 cggctttttc aaaaatatgg tattgataat cctgatatga ataaattgca gtttcatttg
     5221 atgctcgatg agtttttcta atcagaattg gttaattggt tgtaacactg gcagagcatt
     5281 acgctgactt gacgggacgg cgcaagctca tgaccaaaat cccttaacgt gagttttcgt
     5341 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     5401 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     5461 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     5521 caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     5581 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     5641 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     5701 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     5761 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     5821 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     5881 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     5941 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     6001 tcctggcctt ttgctggcct tttgctcaca tgtt
//
