LOCUS       sequence-90552-e         822 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: N1 Cloning Vector, Excitation: 440, Emission: 616.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: dKeima-Red-N1
FEATURES             Location/Qualifiers
     source          1..822
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    17..97
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      99..108
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             105..773
                     /codon_start=1
                     /product="dimeric Keima-Red fluorescent protein"
                     /label=dKeima-Red
                     /translation="MVSVIAKQMTYKVYMSGTVNGHYFEVEGDGKGKPYEGEQTVKLTV
                     TKGGPLPFAWDILSPLFQYGSIPFTKYPEDIPDYVKQSFPEGYTWERTMNFEDGAVCTV
                     SNDSSIQGNCFIYNVKISGTNFPPNGPVMQKKTQGWEPSTERLFARDGMLIGNDYMALK
                     LEGGGHYLCEFKSTYKAKKPVRMPGYHYIDRKLDVTSHNRDYTSVEQCEIAIARHFLLG
                     "
ORIGIN
        1 gtgaaccgtc agatccgcta gcgctaccgg actcagatct cgagctcaag cttcgaattc
       61 tgcagtcgac ggtaccgcgg gcccgggatc caccggtcgc caccatggtg agtgtgatcg
      121 ctaaacaaat gacctacaag gtttatatgt caggcacggt caatggacac tactttgagg
      181 tcgaaggcga tggaaaagga aagccttacg agggagagca gacagtaaag ctcactgtca
      241 ccaagggtgg acctctgcca tttgcttggg atatattatc accactgttt cagtacggaa
      301 gcataccatt caccaagtac cctgaagaca tccctgatta tgtaaagcag tcattccctg
      361 agggatatac atgggagagg accatgaact ttgaagatgg tgcagtgtgt actgtcagca
      421 atgattccag catccaaggc aactgtttca tctacaatgt caaaatctct ggtacgaact
      481 ttcctcccaa tggacctgtt atgcagaaga agacacaggg ctgggaaccc agcactgagc
      541 gtctctttgc acgagatgga atgctgatag gaaacgatta tatggctctg aagttggaag
      601 gaggtggtca ctatttgtgt gaatttaaat ctacttacaa ggcaaagaag cctgtgagga
      661 tgccagggta tcactatatt gaccgcaaac tggatgtaac cagtcacaac agggattaca
      721 catctgttga gcagtgtgaa atagccattg cacgccactt tttgctcggt taagcggccg
      781 cgactctaga tcataatcag ccataccaca tttgtagagg tt
//
