LOCUS       sequence-9056-ex        4164 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4164)
  AUTHORS   Merritt C, Rasoloson D, Ko D, Seydoux G
  TITLE     3' UTRs are the primary regulators of gene expression in the C. 
            elegans germline.
  JOURNAL   Curr Biol. 2008 Oct 14;18(19):1476-82. doi: 
            10.1016/j.cub.2008.08.013. Epub 2008 Sep 25.
  PUBMED    18818082
REFERENCE   2  (bases 1 to 4164)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2008.08.013"; journalName: "Curr Biol"; date: 
            "2008-10-14- 14"; volume: "18"; issue: "19"; pages: "1476-82"
COMMENT     Sequence Label: pCM3.35
FEATURES             Location/Qualifiers
     source          1..4164
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(73..100)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(192..278)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    331..430
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2340..2439)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2551..2570)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2562..3371
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2629..2648)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3514..4102
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4003..4022
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgctagcc aggaagagtt
       61 tgtagaaacg caaaaaggcc atccgtcagg atggccttct gcttagtttg atgcctggca
      121 gtttatggcg ggcgtcctgc ccgccaccct ccgggccgtt gcttcacaac gttcaaatcc
      181 gctcccggcg gatttgtcct actcaggaga gcgttcaccg acaaacaaca gataaaacga
      241 aaggcccagt cttccgactg agcctttcgt tttatttgat gcctggcagt tccctactct
      301 cgcgttaacg ctagcatgga tctcgggccc caaataatga ttttattttg actgatagtg
      361 acctgttcgt tgcaacaaat tgatgagcaa tgctttttta taatgccaag tttgtacaaa
      421 aaagcaggct ccatgtccga taccgtggaa gagctcgttc aacgcgccaa gcttgccgaa
      481 caagccgagc gatacgatga tatggctgcc gccatgaaga aagtaagata aaacatcatt
      541 tacggaggtt tcaaatttac tatttttcag gtgaccgagc agggacagga gctttccaac
      601 gaagagcgta accttctctc tgttgcctac aaaaacgtcg tcggagctcg ccgttcatcg
      661 tggagagtta tctcttccat tgagcaaaag accgagggat ccgagaagaa gcaacaactc
      721 gccaaggaat accgcgtcaa ggtaactaca ccaagcaaga ttttattaat ttcaagaatt
      781 ttagtaactt atttacatta tttcaggttg agcaagaact caacgacatc tgccaagatg
      841 tgctcaagct cctcgacgag ttcctcattg tcaaggctgg agctgctgag tcgaaggtct
      901 tctatctcaa gatgaaagga gattactaca gatacctcgc cgaggtcgct tcagaggatc
      961 gcgctgccgt tgttgagaag tcccagaagg cttaccaggt atttgaaaat aactaaattc
     1021 ttgatgtaaa tggcatgact ttcaggaggc tcttgatatc gctaaggaca agatgcagcc
     1081 aacccaccca attcgccttg gacttgccct caacttctct gtcttctact acgagatctt
     1141 gaacactcca gagcacgctt gccaactcgc gaaacaggct ttcgatgatg ctattgctga
     1201 gctcgacacg ttgaacgagg attcgtacaa ggactcgacc ttgatcatgc aacttctacg
     1261 tgacaatctc actctctgga catctgacgt tggagctgaa gatcaagagc aggagggaaa
     1321 ccaggaagct ggaaactaag tacgatttca aaatgtttta ttgccattat ataaagctga
     1381 cctttttcct cttttccttt cacatcaaat cttgcttcga aaaaatagtt tcggttttca
     1441 gagagctgaa ctccgttctc tttcctcgca tctcttcaac tttcagttat cctccaacat
     1501 ttccggtacg ttagccgaga gagttgccta atcaccgttt ttgttttaga cttaacgtat
     1561 tcaccaatac tccatacaac actgcgcaat agcatcatga aagaaaattc ggtcttcaag
     1621 tgctctagat ctccaagcca taattctctt catgctctat ttcgctgtaa ttcactccgt
     1681 cactattgtc attcctaaaa tggcgtattg ttctatgatt attatgtgct gtaaagtata
     1741 atgaaccccc atcttccatt tttctcttat tatccccgat ctttattttt agatcttttt
     1801 gaacataaat gtttggtgaa cttttccggt tgtccgaatt tcatcacaac tttccaggag
     1861 tttttcgagt aatggaatgg aatttatttt cagatttcga tgaaggagca taaataggat
     1921 aaaacatttg cattaacctc ttagaaggtc tcaacacgaa aaattaaatg aaatgtgaaa
     1981 aagtgtgcgc ctttaagctg ccgaaaattt tttgatagtt tcttccacga ttcgttaaaa
     2041 attaagtgtt ttatggtaac atttgagtgt tcttttgaaa ataaatatat tctttgtcga
     2101 aaaactatga aacatcgata aaaattccgc agcaatagaa gtttaaaatt acagtactct
     2161 ttaaagactc acaccttttt gcgttcaaca aatattagac gtgtcgagac taagtaccgt
     2221 acttttggcg caaaaagctc aaaatttcgc tcctgggcaa tataattttc ggaaaaaaac
     2281 ttaattctgg agacttcatg tgttttcaca gatcatgacg agaccgattg agaaacatta
     2341 cccagctttc ttgtacaaag tgggcattat aagaaagcat tgcttatcaa tttgttgcaa
     2401 cgaacaggtc actatcagtc aaaataaaat cattatttgc catccagctg cagctctggc
     2461 ccgtgtctca aaatctctga tgttacattg cacaagataa aaatatatca tcatgaacaa
     2521 taaaactgtc tgcttacata aacagtaata caaggggtgt tatgagccat attcaacggg
     2581 aaacgtcgag gccgcgatta aattccaaca tggatgctga tttatatggg tataaatggg
     2641 ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg cttgtatggg aagcccgatg
     2701 cgccagagtt gtttctgaaa catggcaaag gtagcgttgc caatgatgtt acagatgaga
     2761 tggtcagact aaactggctg acggaattta tgcctcttcc gaccatcaag cattttatcc
     2821 gtactcctga tgatgcatgg ttactcacca ctgcgatccc cggaaaaaca gcattccagg
     2881 tattagaaga atatcctgat tcaggtgaaa atattgttga tgcgctggca gtgttcctgc
     2941 gccggttgca ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc
     3001 tcgctcaggc gcaatcacga atgaataacg gtttggttga tgcgagtgat tttgatgacg
     3061 agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat gcataaactt ttgccattct
     3121 caccggattc agtcgtcact catggtgatt tctcacttga taaccttatt tttgacgagg
     3181 ggaaattaat aggttgtatt gatgttggac gagtcggaat cgcagaccga taccaggatc
     3241 ttgccatcct atggaactgc ctcggtgagt tttctccttc attacagaaa cggctttttc
     3301 aaaaatatgg tattgataat cctgatatga ataaattgca gtttcatttg atgctcgatg
     3361 agtttttcta atcagaattg gttaattggt tgtaacactg gcagagcatt acgctgactt
     3421 gacgggacgg cgcaagctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     3481 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     3541 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     3601 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     3661 ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     3721 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     3781 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     3841 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     3901 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     3961 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     4021 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     4081 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     4141 ttgctggcct tttgctcaca tgtt
//
