LOCUS       sequence-90560-e        1034 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of mouse DIRAS1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   Fritsch R, de Krijger I, Fritsch K, George R, Reason B, Kumar MS, 
            Diefenbacher M, Stamp G, Downward J
  TITLE     RAS and RHO families of GTPases directly regulate distinct 
            phosphoinositide 3-kinase isoforms.
  JOURNAL   Cell. 2013 May 23;153(5):1050-63. doi: 10.1016/j.cell.2013.04.031.
  PUBMED    23706742
REFERENCE   2  (bases 1 to 1034)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.04"; journalName: "Cell"; date: "2013-05-23- 
            23"; volume: "153"; issue: "5"; pages: "1050-63"
COMMENT     Sequence Label: DIRAS1
FEATURES             Location/Qualifiers
     source          1..1034
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             9..26
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(679..701)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(846..864)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        932..1034
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aatcggatct ggttccgcgt ggatccccag aacagagcaa tgactaccgt gtggtcgtgt
       61 tcggcgcagg cggcgtgggc aagagctcgc tggtgctccg ctttgtaaag gggacgtttc
      121 gtgacaccta catccctacc atagaggaca cgtaccggca ggtgatcagc tgtgacaaga
      181 gcgtgtgcac actgcagatc acggacacca ccggcagcca ccagttcccg gccatgcagc
      241 ggctgtccat ctccaagggc cacgccttta tcctggtgtt ctcggtgacc agcaagcagt
      301 cgctggatga gctgagcccc atctacaagt tgatcgtgca gatcaagggc agcgtggagg
      361 acatccccat catgctggtg gggaacaagt gtgatgagac gcagcgggag gtgcacacgc
      421 gcgaggcgca ggccgtggca caggagtgga agtgcgcctt catggaaacc tcagcgaaga
      481 tgaactacaa cgtgaaggag ctgttccagg agctgctgac gctcgagacg cgccggagcg
      541 tcagcctcag cgtggacggc aaacgctcca gcaagcagaa gagggctgac cgcatcaagg
      601 gcaagtgcgc gctcatgtga gaattcatcg tgactgactg acgatctgcc tcgcgcgttt
      661 cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct
      721 gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg
      781 tcggggcgca gccatgaccc agtcacgtag cgatagcgga gtgtataatt cttgaagacg
      841 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
      901 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
      961 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     1021 ttgaaaaagg aaga
//
