LOCUS       sequence-90561-e         863 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: C1 Cloning Vector, Excitation: 580, Emission: 515.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 863)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: mKind1-C1
FEATURES             Location/Qualifiers
     source          1..863
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      33..42
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             39..734
                     /codon_start=1
                     /product="FusionRed fluorescent protein derivative that 
                     shows reversible photoswitching with visible light and fast
                     OFF-switching kinetics"
                     /label=rsFusionRed2
                     /translation="MVSELIKENMPMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     VVEGGPLPFAFDILATSFMYGSKTFIKHPPGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKVRGVNFPANGPVMQKKTLGWEAHTETMYPADGGLEGACDMAL
                     KLVGGGHLICNLETTYRSKKPATNLKMPGVYNVDHRLERIKEADDETYVEQHEVAVARY
                     STGGAGDGGK"
     misc_feature    747..812
                     /label=MCS
                     /note="multiple cloning site"
ORIGIN
        1 gtgaaccgtc agatccgcta gcgctaccgg tcgccaccat ggtgagcgag ctgattaagg
       61 agaacatgcc catgaagctg tacatggagg gcaccgtgaa caaccaccac ttcaagtgca
      121 catccgaggg cgaaggcaag ccctacgagg gcacccagac catgagaatc aaggtcgtcg
      181 agggcggccc tctccccttc gccttcgaca tcctggctac cagcttcatg tacggcagca
      241 aaaccttcat caagcaccct ccgggcatcc ccgacttctt taagcagtcc ttccctgagg
      301 gcttcacatg ggagagagtc accacatacg aagacggggg cgtgctgacc gctacccagg
      361 acaccagcct ccaggacggc tgcctcatct acaacgtcaa ggttagaggg gtgaacttcc
      421 cagccaacgg ccctgtgatg cagaagaaaa cactcggctg ggaggcccac accgagacga
      481 tgtaccccgc tgacggcggc ctggaaggcg catgtgacat ggccctgaag ctcgtgggcg
      541 ggggccacct gatctgcaac cttgagacca catacagatc caagaaaccc gctacgaacc
      601 tcaagatgcc cggcgtctac aacgtggacc acagactgga aagaatcaag gaggccgacg
      661 atgagaccta cgtcgagcag cacgaggtgg ctgtggccag atactctact ggtggcgctg
      721 gtgatggagg taaaggtgga ggaggttccg gactcagatc tcgagctcaa gcttcgaatt
      781 ctgcagtcga cggtaccgcg ggcccgggat ccaccggatc tagataactg atcataatca
      841 gccataccac atttgtagag gtt
//
