LOCUS       sequence-9057-ex        4659 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4659)
  AUTHORS   Merritt C, Rasoloson D, Ko D, Seydoux G
  TITLE     3' UTRs are the primary regulators of gene expression in the C. 
            elegans germline.
  JOURNAL   Curr Biol. 2008 Oct 14;18(19):1476-82. doi: 
            10.1016/j.cub.2008.08.013. Epub 2008 Sep 25.
  PUBMED    18818082
REFERENCE   2  (bases 1 to 4659)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2008.08.013"; journalName: "Curr Biol"; date: 
            "2008-10-14- 14"; volume: "18"; issue: "19"; pages: "1476-82"
COMMENT     Sequence Label: pCM3.08
FEATURES             Location/Qualifiers
     source          1..4659
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(73..100)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(192..278)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    331..430
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2835..2934)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3046..3065)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             3057..3866
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3124..3143)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      4009..4597
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4498..4517
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgctagcc aggaagagtt
       61 tgtagaaacg caaaaaggcc atccgtcagg atggccttct gcttagtttg atgcctggca
      121 gtttatggcg ggcgtcctgc ccgccaccct ccgggccgtt gcttcacaac gttcaaatcc
      181 gctcccggcg gatttgtcct actcaggaga gcgttcaccg acaaacaaca gataaaacga
      241 aaggcccagt cttccgactg agcctttcgt tttatttgat gcctggcagt tccctactct
      301 cgcgttaacg ctagcatgga tctcgggccc caaataatga ttttattttg actgatagtg
      361 acctgttcgt tgcaacaaat tgatgagcaa tgctttttta taatgccaag tttgtacaaa
      421 aaagcaggct ccatggctca aacaaagccg attgccgagc aaatggctgc actcaacaac
      481 tcagatgaca cgtcgttcgc tgccgatcga tcgaacagtc ttctgaatgc gacgtgcccg
      541 gcgagaattc aaaattcagt agatcaacgg aaaatcaatc gatcattcaa tgattcgctg
      601 tcgtccggat atagtggaaa atggcttcgt ccaaagcgtg aagcgctcaa gatcactcca
      661 ttggctcaga ttgacgaggc gccggcaact aaaagacata gctcggcgaa ggataaggtt
      721 ttgatttaaa aaaaaaactg aaaaatatgc aaaatcgatt ttttttccag cacacagaat
      781 acaaaacgcg actttgtgat gcgttccgcc gtgaaggata ctgcccgtac aacgacaatt
      841 gcacatatgc tcacggacaa gatgagctga gagttccggt gtgtctgaag cctggaaatc
      901 tttggaaaat ttttttttca gagacgccgc caagagtatt attcccgaga tccaccacgt
      961 gagcgccgtg attctcgttc tagacgagac gacgtggata caacaatcaa tcgatcgagt
     1021 tcttcagcat cgaagcatca tgatgaggtg ctgatcggat ggaaattcgc ttttttgaca
     1081 aattttcgca gttttttcgc taaatttcat ttaaaatatg cttattaaca cattatatca
     1141 ctatttagac gaatatcttg tagaaatgtt gggttttgtt cgaattttct ctactttttg
     1201 agttgcttca cgaagaaatt gtgttctctt tcattgtaca gaagaatcta tgttttacaa
     1261 gggaaaaaag taagatggtg caacattttc tcgtgaatcg actctaaaag tagagaatta
     1321 tcgaacaaaa ccaacatttc tacaagatat tcgcctaagt actgatatta tgtgataata
     1381 aatgaaattt agcgagaaaa tactgcgaaa acgccttttc agacccaaaa ccgcctaaat
     1441 ttctcgaatt ttcagggcta aatcatttta aaactccaat ttcgctcaat tttccgtgtc
     1501 gagacggtcc gaagttgatt ttctggaatt atcatccttg ttttgcatct tttagtagtt
     1561 tatctcaaat ttttcccgcc tgcagacaga ttatgtgaga taaactacca aaatgtgcat
     1621 taaattatga taattccaaa aaaagtcaac tttcggccga tgcggcacga aaaattgcca
     1681 aaattcgagg tttttagcta aaattatcaa aactttggac aaaaatcatc cattgcgcag
     1741 cgaatttttg gggttatacg ttcaaaatgt ttattttaaa atgaaattta acgagaaaat
     1801 ataatatact gcgaaaacgg cttctcagac caaaaatcgc ccaaaactcg aattttcagg
     1861 gctaaattgg ctaaaagtct tcatttttgg caagttctcc gtgtggcgac ggaaaaaaag
     1921 ccaatttctg gcaaaattga agattttcag ctaaaattaa ccctgttttt caaaattttg
     1981 aacaaaaatc gtcaatttca gcgaattttt gaggttttac gttgcaaaaa tccttatttt
     2041 taagtgaaat ttagggaaaa tattacaata ttttacttcc agaatcggag acccagcaac
     2101 aaccacggaa gctcgaatcg tcgtcagatt tgtcacaatt tcgagagagg aaactgcaga
     2161 tatggtccaa ggttctagga aatctggaat ttttattaaa aataaataaa ataaattttc
     2221 ctttcagatg ccgcttcatt cacgtcgaac aaatgcaaca tttcaatgcg aatgcgacgg
     2281 tttacgcgcc accttcttcc gattgtccgc cgccgattgc ctactaccat catcatccac
     2341 aacatcagca acaattcctg ccatttccaa tgccatattt cttggctcca ccgccgcaag
     2401 ctcaacaagg agctcctttt ccagtgcaat atattccaca gcaacatgat ttgatgaata
     2461 gccagccaat gtatgcacca atggcaccga catactacta tcaaccaatt aattcgaatg
     2521 gcatgcccat gatggatgtg actattgatc cgaatgccac gggcggtgcg tttgaagtgt
     2581 tccccgatgg attcttctct cagccaccac caactattat ttcctaattt tgccgtattt
     2641 tccatatttt gttttgtata tttatccact caccccctct ctttgtcctg tgaatgaact
     2701 tgtgccaaaa aagccaaaaa taatttactt tttttaaaga catattaaaa cttgaaaact
     2761 tggaacaata cggtgcgaaa gcgaaacaga gacagaaaaa tgaacaaaga gatccgtgaa
     2821 aagtgaacga tgatacccag ctttcttgta caaagtgggc attataagaa agcattgctt
     2881 atcaatttgt tgcaacgaac aggtcactat cagtcaaaat aaaatcatta tttgccatcc
     2941 agctgcagct ctggcccgtg tctcaaaatc tctgatgtta cattgcacaa gataaaaata
     3001 tatcatcatg aacaataaaa ctgtctgctt acataaacag taatacaagg ggtgttatga
     3061 gccatattca acgggaaacg tcgaggccgc gattaaattc caacatggat gctgatttat
     3121 atgggtataa atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc tatcgcttgt
     3181 atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc gttgccaatg
     3241 atgttacaga tgagatggtc agactaaact ggctgacgga atttatgcct cttccgacca
     3301 tcaagcattt tatccgtact cctgatgatg catggttact caccactgcg atccccggaa
     3361 aaacagcatt ccaggtatta gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc
     3421 tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg taattgtcct tttaacagcg
     3481 atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa taacggtttg gttgatgcga
     3541 gtgattttga tgacgagcgt aatggctggc ctgttgaaca agtctggaaa gaaatgcata
     3601 aacttttgcc attctcaccg gattcagtcg tcactcatgg tgatttctca cttgataacc
     3661 ttatttttga cgaggggaaa ttaataggtt gtattgatgt tggacgagtc ggaatcgcag
     3721 accgatacca ggatcttgcc atcctatgga actgcctcgg tgagttttct ccttcattac
     3781 agaaacggct ttttcaaaaa tatggtattg ataatcctga tatgaataaa ttgcagtttc
     3841 atttgatgct cgatgagttt ttctaatcag aattggttaa ttggttgtaa cactggcaga
     3901 gcattacgct gacttgacgg gacggcgcaa gctcatgacc aaaatccctt aacgtgagtt
     3961 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     4021 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     4081 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     4141 gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     4201 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     4261 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     4321 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     4381 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     4441 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     4501 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     4561 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     4621 acggttcctg gccttttgct ggccttttgc tcacatgtt
//
