LOCUS       sequence-90578-e         855 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: N1 Cloning Vector, Excitation: 507 / 583, Emission: 
            517 / 593.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 855)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: mKikGR-N1
FEATURES             Location/Qualifiers
     source          1..855
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    17..97
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      99..108
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             105..806
                     /codon_start=1
                     /product="monomeric photoconvertible Kikume green-to-red 
                     fluorescent protein"
                     /label=mKikGR1
                     /translation="MVSVITSEMKIELRMEGSVNGHKFVITGKGSGRPYEGTQTVDLTV
                     IEGGPLPFAFDILTTAFHYGNRVFVEYPEEIVDYFKQLFPEGYSWERSMSYEDGGICLA
                     TNNITMKKDGSNTFVNEIRFDGTNFPANGPVMQRKTVKWEPSTEKMYVRDGVLKGDVEM
                     ALLLEGGGHYRCDFRTTYKAKKVVQLPDYHYVDHQMEITSHDKDYNKVKAYEHAKAYSG
                     TYRGAKYEFEA"
ORIGIN
        1 gtgaaccgtc agatccgcta gcgctaccgg actcagatct cgagctcaag cttcgaattc
       61 tgcagtcgac ggtaccgcgg gcccgggatc caccggtcgc caccatggtg agtgtgatta
      121 catcagaaat gaagatcgag ctgcgtatgg aaggctctgt aaacgggcac aagttcgtga
      181 ttacagggaa aggaagtggc agaccttacg agggaacaca gactgtggac ctgacagtca
      241 tagagggcgg acctcttcct tttgctttcg atatcctgac aacagcattc cattacggca
      301 accgggtatt tgtcgaatac ccagaagaaa tagtagacta cttcaagcag ttgtttcctg
      361 agggttattc ttgggaacga agcatgagtt acgaagacgg gggaatttgc ctcgccacaa
      421 acaatataac gatgaagaaa gacggcagca acacttttgt caatgaaatt cgatttgatg
      481 gtacaaactt tcctgccaat ggtccagtta tgcagaggaa gaccgtcaaa tgggagccat
      541 ccactgagaa aatgtatgtg cgtgatggag tgctgaaggg tgatgttgaa atggctctgt
      601 tgcttgaagg aggtggccat taccgatgtg acttcagaac tacttacaaa gcaaagaagg
      661 ttgtccagtt gccagactat cactacgtgg atcatcaaat ggagataaca agccatgaca
      721 aggattacaa caaggttaag gcgtatgaac atgctaaggc ttattccggg acctacaggg
      781 gcgccaagta tgaatttgaa gcctaagcgg ccgcgactct agatcataat cagccatacc
      841 acatttgtag aggtt
//
