LOCUS       sequence-90581-e         833 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: C1 Cloning Vector, Excitation: 590, Emission: 649.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Kremers GJ, Hazelwood KL, Murphy CS, Davidson MW, Piston DW
  TITLE     Photoconversion in orange and red fluorescent proteins.
  JOURNAL   Nat Methods. 2009 May;6(5):355-8. doi: 10.1038/nmeth.1319. Epub 2009
            Apr 12.
  PUBMED    19363494
REFERENCE   2  (bases 1 to 833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1319"; journalName: "Nat Methods"; date: "2009-05"; 
            volume: "6"; issue: "5"; pages: "355-8"
COMMENT     Sequence Label: mPlum-C1
FEATURES             Location/Qualifiers
     source          1..833
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             39..716
                     /codon_start=1
                     /product="far-red monomeric derivative of DsRed fluorescent
                     protein (Wang et al., 2004)"
                     /label=mPlum
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKEHMEGSVNGHEFEIEGEGEGRPYEGTQTA
                     RLKVTKGGPLPFAWDILSPQIMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGEFIYKVKVRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEMK
                     MRLRLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRH
                     STGA"
     primer_bind     440..459
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     634..657
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     misc_feature    717..782
                     /label=MCS
                     /note="multiple cloning site"
ORIGIN
        1 gtgaaccgtc agatccgcta gcgctaccgg tggctaccat ggtgagcaag ggcgaggagg
       61 tcatcaagga gttcatgcgc ttcaaggagc acatggaggg ctccgtgaac ggccacgagt
      121 tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaggctga
      181 aggtgaccaa gggtggcccc ctgcccttcg cctgggacat cctgtcccct cagatcatgt
      241 acggctccaa ggcctacgtg aagcaccccg ccgacatccc cgactacttg aagctgtcct
      301 tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg
      361 tgacccagga ctcctccctg caggacggcg agttcatcta caaggtgaag gtgcgcggca
      421 ccaacttccc ctccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctcct
      481 ccgagcggat gtaccccgag gacggcgccc tgaagggcga gatgaagatg aggctgaggc
      541 tgaaggacgg cggccactac gacgccgagg tcaagaccac ctacatggcc aagaagcccg
      601 tgcagctgcc cggcgcctac aagaccgaca tcaagctgga catcacctcc cacaacgagg
      661 actacaccat cgtggaacag tacgagcgcg ccgagggccg ccactccacc ggcgcctccg
      721 gactcagatc tcgagctcaa gcttcgaatt ctgcagtcga cggtaccgcg ggcccgggat
      781 ccaccggatc tagataactg atcataatca gccataccac atttgtagag gtt
//
