LOCUS       sequence-90586-e         833 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: N1 Cloning Vector, Excitation: 508 / 572, Emission: 
            518 / 580.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Kremers GJ, Hazelwood KL, Murphy CS, Davidson MW, Piston DW
  TITLE     Photoconversion in orange and red fluorescent proteins.
  JOURNAL   Nat Methods. 2009 May;6(5):355-8. doi: 10.1038/nmeth.1319. Epub 2009
            Apr 12.
  PUBMED    19363494
REFERENCE   2  (bases 1 to 833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1319"; journalName: "Nat Methods"; date: "2009-05"; 
            volume: "6"; issue: "5"; pages: "355-8"
COMMENT     Sequence Label: Kaede-N1
FEATURES             Location/Qualifiers
     source          1..833
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    18..98
                     /label=MCS
                     /note="multiple cloning site"
     CDS             106..783
                     /codon_start=1
                     /product="Kaede green-to-red photoconvertible fluorescent 
                     protein (Ando et al., 2002)"
                     /label=Kaede
                     /translation="MSLIKPEMKIKLLMEGNVNGHQFVIEGDGKGHPFEGKQSMDLVVK
                     EGAPLPFAYDILTTAFHYGNRVFAKYPDHIPDYFKQSFPKGFSWERSLMFEDGGVCIAT
                     NDITLKGDTFFNKVRFDGVNFPPNGPVMQKKTLKWEASTEKMYLRDGVLTGDITMALLL
                     KGDVHYRCDFRTTYKSRQEGVKLPGYHFVDHCISILRHDKDYNEVKLYEHAVAHSGLPD
                     NVK"
ORIGIN
        1 agtgaaccgt cagatccgct agcgctaccg gactcagatc tcgagctcaa gcttcgaatt
       61 ctgcagtcga cggtaccgcg ggcccgggat ccaccggtcg ccaccatgag tctgattaaa
      121 ccagaaatga agatcaagct gcttatggaa ggcaatgtaa acgggcacca gtttgttatt
      181 gagggagatg gaaaaggcca tccttttgag ggaaaacaga gtatggacct tgtagtcaaa
      241 gaaggcgcac ctctcccttt tgcctacgat atcttgacaa cagcattcca ttatggtaac
      301 agggtttttg ctaaataccc agaccatata ccagactact tcaagcagtc gtttcccaaa
      361 gggttttctt gggagcgaag cctgatgttc gaggacgggg gcgtttgcat cgctacaaat
      421 gacataacac tgaaaggaga cacttttttt aacaaagttc gatttgatgg cgtaaacttt
      481 cccccaaatg gtcctgttat gcagaagaag actctgaaat gggaggcatc cactgagaaa
      541 atgtatttgc gtgatggagt gttgacgggc gatattacaa tggctctgct gcttaaagga
      601 gatgtccatt accgatgtga cttcagaact acttacaaat ctaggcagga gggtgtcaag
      661 ttgccaggat atcactttgt cgatcactgc atcagcatat tgaggcatga caaagactac
      721 aacgaggtta agctgtatga gcacgctgtt gcccattctg gattgccgga caacgtcaag
      781 taaagcggcc gcgactctag atcataatca gccataccac atttgtagag gtt
//
