LOCUS       sequence-90587-e         797 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: C1 Cloning Vector, Excitation: 548, Emission: 561.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 797)
  AUTHORS   Karasawa S, Araki T, Nagai T, Mizuno H, Miyawaki A
  TITLE     Cyan-emitting and orange-emitting fluorescent proteins as a 
            donor/acceptor pair for fluorescence resonance energy transfer.
  JOURNAL   Biochem J. 2004 Jul 1. 381(Pt 1):307-12.
  PUBMED    15065984
REFERENCE   2  (bases 1 to 797)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochem J. 
            2004 Jul 1. 381(Pt 1):307-12."
COMMENT     Sequence Label: mKusabira Orange-C1 (mKO)
FEATURES             Location/Qualifiers
     source          1..797
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      26..35
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             32..685
                     /codon_start=1
                     /product="monomeric Kusabira-Orange fluorescent protein 
                     (Karasawa et al., 2004)"
                     /label=mKO
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAKGGPMPFAFDLVSHVFCYGHRPFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQFKTTYKAAKKILKMPGSHYISHRLVRKTEGNITELVEDAVAHS"
ORIGIN
        1 gtgaaccgtc agatccgcta gcattgccac catggtgagt gtgattaaac cagagatgaa
       61 gatgaggtac tacatggacg gctccgtcaa tgggcatgag ttcacaattg aaggtgaagg
      121 cacaggcaga ccttacgagg gacatcaaga gatgacacta cgcgtcacaa tggccaaggg
      181 cgggccaatg cctttcgcgt ttgacttagt gtcacacgtg ttctgttacg gccacagacc
      241 ttttactaaa tatccagaag agataccaga ctatttcaaa caagcatttc ctgaaggcct
      301 gtcatgggaa aggtcgttgg agttcgaaga tggtgggtcc gcttcagtca gtgcgcatat
      361 aagccttaga ggaaacacct tctaccacaa atccaaattt actggggtta actttcctgc
      421 cgatggtcct atcatgcaaa accaaagtgt tgattgggag ccatcaaccg agaaaattac
      481 tgccagcgac ggagttctga agggtgatgt tacgatgtac ctaaaacttg aaggaggcgg
      541 caatcacaaa tgccaattca agactactta caaggcggca aaaaagattc ttaaaatgcc
      601 aggaagccat tacatcagcc atcgcctcgt caggaaaacc gaaggcaaca ttactgagct
      661 ggtagaagat gcagtagctc attccggatc ctcaggtacc ggaactgcag cagagaattc
      721 gggaaactcg agaacaaagc ttgaataagc ggccgcgact ctagatcata atcagccata
      781 ccacatttgt agaggtt
//
