LOCUS       sequence-90588-e         821 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: C1 Cloning Vector, Excitation: 440, Emission: 620.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: mKeima-Red-C1
FEATURES             Location/Qualifiers
     source          1..821
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      33..42
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(39..41,42..44,45..704)
                     /codon_start=1
                     /product="monomeric Keima-Red fluorescent protein, also 
                     known as mKeima"
                     /label=mKeima-Red
                     /translation="MVSVIAKQMTYKVYMSGTVNGHYFEVEGDGKGKPYEGEQTVKLTV
                     TKGGPLPFAWDILSPQLQYGSIPFTKYPEDIPDYFKQSFPEGYTWERSMNFEDGAVCTV
                     SNDSSIQGNCFIYNVKISGENFPPNGPVMQKKTQGWEPSTERLFARDGMLIGNDYMALK
                     LEGGGHYLCEFKSTYKAKKPVRMPGRHEIDRKLDVTSHNRDYTSVEQCEIAIARHSLLG
                     "
     misc_feature    705..770
                     /label=MCS
                     /note="multiple cloning site"
ORIGIN
        1 gtgaaccgtc agatccgcta gcgctaccgg tcgccaccat ggtgagtgtg atcgctaaac
       61 aaatgaccta caaggtttat atgtcaggca cggtcaatgg acactacttt gaggtcgaag
      121 gcgatggaaa aggaaagcct tacgagggag agcagacagt aaagctcact gtcaccaagg
      181 gtggacctct gccatttgct tgggatattt tatcaccaca gcttcagtac ggaagcatac
      241 cattcaccaa gtaccctgaa gacatccctg attatttcaa gcagtcattc cctgagggat
      301 atacatggga gaggagcatg aactttgaag atggtgcagt gtgtactgtc agcaatgatt
      361 ccagcatcca aggcaactgt ttcatctaca atgtcaaaat ctctggtgag aactttcctc
      421 ccaatggacc tgttatgcag aagaagacac agggctggga acccagcact gagcgtctct
      481 ttgcacgaga tggaatgctg ataggaaacg attatatggc tctgaagttg gaaggaggtg
      541 gtcactattt gtgtgaattt aaatctactt acaaggcaaa gaagcctgtg aggatgccag
      601 ggcgccacga gattgaccgc aaactggatg taaccagtca caacagggat tacacatctg
      661 ttgagcagtg tgaaatagcc attgcacgcc actctttgct cggttccgga ctcagatctc
      721 gagctcaagc ttcgaattct gcagtcgacg gtaccgcggg cccgggatcc accggatcta
      781 gataactgat cataatcagc cataccacat ttgtagaggt t
//
