LOCUS       sequence-90590-e         833 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin, Excitation: 558, Emission: 605.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: mRuby-Lifeact-7
FEATURES             Location/Qualifiers
     source          1..833
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      23..32
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             29..79
                     /codon_start=1
                     /product="peptide that binds filamentous actin (Riedl et 
                     al., 2008)"
                     /label=Lifeact
                     /note="first 17 amino acids of budding yeast Abp140"
                     /translation="MGVADLIKKFESISKEE"
     CDS             101..781
                     /codon_start=1
                     /product="monomeric variant of the red fluorescent protein 
                     eqFP611 (Kredel et al., 2009)"
                     /label=mRuby
                     /translation="MNSLIKENMRMKVVLEGSVNGHQFKCTGEGEGNPYMGTQTMRIKV
                     IEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGGVITV
                     MQDTSLEDGCLVYHAQVRGVNFPSNGAVMQKKTKGWEPNTEMMYPADGGLRGYTHMALK
                     VDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAVAKFA
                     GLGGG"
ORIGIN
        1 gtgaaccgtc agatccgcta gcgccaccat gggcgtggcc gacttgatca agaagttcga
       61 gtccatctcc aaggaggagg gggatccacc ggtcgccacc atgaacagcc tgatcaaaga
      121 aaacatgcgg atgaaggtgg tgctggaagg cagcgtgaac ggccaccagt tcaagtgcac
      181 cggcgagggc gagggcaacc cctacatggg cacccagacc atgcggatca aagtgatcga
      241 gggcggacct ctgcccttcg ccttcgacat cctggccaca tccttcatgt acggcagccg
      301 gaccttcatc aagtacccca agggcatccc cgatttcttc aagcagagct tccccgaggg
      361 cttcacctgg gagagagtga ccagatacga ggacggcggc gtgatcaccg tgatgcagga
      421 caccagcctg gaagatggct gcctggtgta ccatgcccag gtcaggggcg tgaattttcc
      481 cagcaacggc gccgtgatgc agaagaaaac caagggctgg gagcccaaca ccgagatgat
      541 gtaccccgct gacggcggac tgagaggcta cacccacatg gccctgaagg tggacggcgg
      601 agggcacctg agctgcagct tcgtgaccac ctaccgatcc aagaaaaccg tgggcaacat
      661 caagatgccc ggcatccacg ccgtggacca ccggctggaa aggctggaag agtccgacaa
      721 cgagatgttc gtggtgcagc gggagcacgc cgtggccaag ttcgccggcc tgggcggagg
      781 gtaagcggcc gcgactctag atcataatca gccataccac atttgtagag gtt
//
