LOCUS       sequence-90591-e         834 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: N1 Cloning Vector, Excitation: 505 / 569, Emission: 
            516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 834)
  AUTHORS   McKinney SA, Murphy CS, Hazelwood KL, Davidson MW, Looger LL
  TITLE     A bright and photostable photoconvertible fluorescent protein.
  JOURNAL   Nat Methods. 2009 Feb . 6(2):131-3.
  PUBMED    19169260
REFERENCE   2  (bases 1 to 834)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2009 Feb . 6(2):131-3."
COMMENT     Sequence Label: mEos2-N1
FEATURES             Location/Qualifiers
     source          1..834
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    17..97
                     /label=MCS
                     /note="multiple cloning site"
     CDS             105..785
                     /codon_start=1
                     /product="monomeric variant of green-to-red photoswitchable
                     fluorescent protein EosFP (McKinney et al., 2009)"
                     /label=mEos2
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIHMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
ORIGIN
        1 gtgaaccgtc agatccgcta gcgctaccgg actcagatct cgagctcaag cttcgaattc
       61 tgcagtcgac ggtaccgcgg gcccgggatc caccggtcgc caccatgagt gcgattaagc
      121 cagacatgaa gatcaaactc cgtatggaag gcaacgtaaa cgggcaccac tttgtgatcg
      181 acggagatgg tacaggcaag ccttttgagg gaaaacagag tatggatctt gaagtcaaag
      241 agggcggacc tctgcctttt gcctttgata tcctgaccac tgcattccat tacggcaaca
      301 gggtattcgc caaatatcca gacaacatac aagactattt taagcagtcg tttcctaagg
      361 ggtattcgtg ggaacgaagc ttgactttcg aagacggggg catttgcatt gccagaaacg
      421 acataacaat ggaaggggac actttctata ataaagttcg attttatggt accaactttc
      481 ccgccaatgg tccagttatg cagaagaaga cgctgaaatg ggagccctcc actgagaaaa
      541 tgtatgtgcg tgatggagtg ctgacgggtg atattcatat ggctttgttg cttgaaggaa
      601 atgcccatta ccgatgtgac ttcagaacta cttacaaagc taaggagaag ggtgtcaagt
      661 taccaggcta ccactttgtg gaccactgca ttgagatttt aagccatgac aaagattaca
      721 acaaggttaa gctgtatgag catgctgttg ctcattctgg attgcctgac aatgccagac
      781 gataagcggc cgcgactcta gatcataatc agccatacca catttgtaga ggtt
//
