LOCUS       sequence-90594-e         857 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: C1 Cloning Vector, Excitation: 548, Emission: 561.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 857)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 857)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: mKOet-C1
FEATURES             Location/Qualifiers
     source          1..857
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      33..42
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             66..716
                     /codon_start=1
                     /product="monomeric Kusabira-Orange fluorescent protein 
                     (Karasawa et al., 2004)"
                     /label=mKO
                     /translation="MAVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRVT
                     MAKGGPMPFAFDLVSHVFCYGHRPFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSAS
                     VSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMYL
                     KLEGGGNHKCQFKTTYKAAKKILKMPGSHYISHRLVRKTEGNITELVEDAVAHS"
     misc_feature    741..806
                     /label=MCS
                     /note="multiple cloning site"
ORIGIN
        1 gtgaaccgtc agatccgcta gcgctaccgg tcgccaccat ggtgagcaag ggcgaggaga
       61 ataacatggc cgtgattaaa ccagagatga agatgaggta ctacatggac ggctccgtca
      121 atgggcatga gttcacaatt gaaggtgaag gcacaggcag accttacgag ggacatcaag
      181 agatgacact acgcgtcaca atggccaagg gcgggccaat gcctttcgcg tttgacttag
      241 tgtcacacgt gttctgttac ggccacagac cttttactaa atatccagaa gagataccag
      301 actatttcaa acaagcattt cctgaaggcc tgtcatggga aaggtcgttg gagttcgaag
      361 atggtgggtc cgcttcagtc agtgcgcata taagccttag aggaaacacc ttctaccaca
      421 aatccaaatt tactggggtt aactttcctg ccgatggtcc tatcatgcaa aaccaaagtg
      481 ttgattggga gccatcaacc gagaaaatta ctgccagcga cggagttctg aagggtgatg
      541 ttacgatgta cctaaaactt gaaggaggcg gcaatcacaa atgccaattc aagactactt
      601 acaaggcggc aaaaaagatt cttaaaatgc caggaagcca ttacatcagc catcgcctcg
      661 tcaggaaaac cgaaggcaac attactgagc tggtagaaga tgcagtagct cattccggcg
      721 gcatggacga gctgtacaag tccggactca gatctcgagc tcaagcttcg aattctgcag
      781 tcgacggtac cgcgggcccg ggatccaccg gatctagata actgatcata atcagccata
      841 ccacatttgt agaggtt
//
