LOCUS       sequence-90595-e         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin, Excitation: 507 / 572, Emission: 516 / 580.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: mEos3.2-Lifeact-7
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      23..32
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             29..79
                     /codon_start=1
                     /product="peptide that binds filamentous actin (Riedl et 
                     al., 2008)"
                     /label=Lifeact
                     /note="first 17 amino acids of budding yeast Abp140"
                     /translation="MGVADLIKKFESISKEE"
     CDS             101..781
                     /codon_start=1
                     /product="improved monomeric variant of green-to-red 
                     photoswitchable fluorescent protein EosFP (Zhang et al., 
                     2012)"
                     /label=mEos3.2
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIEMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGAHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
ORIGIN
        1 gtgaaccgtc agatccgcta gcgccaccat gggcgtggcc gacttgatca agaagttcga
       61 gtccatctcc aaggaggagg gggatccacc ggtcgccacc atgagtgcga ttaagccaga
      121 catgaagatc aaactccgta tggaaggcaa cgtaaacggg caccactttg tgatcgacgg
      181 agatggtaca ggcaagcctt ttgagggaaa acagagtatg gatcttgaag tcaaagaggg
      241 cggacctctg ccttttgcct ttgatatcct gaccactgca ttccattacg gcaacagggt
      301 attcgccaaa tatccagaca acatacaaga ctattttaag cagtcgtttc ctaaggggta
      361 ttcgtgggaa cgaagcttga ctttcgaaga cgggggcatt tgcaacgcca gaaacgacat
      421 aacaatggaa ggggacactt tctataataa agttcgattt tatggtacca actttcccgc
      481 caatggtcca gttatgcaga agaagacgct gaaatgggag ccctccactg agaaaatgta
      541 tgtgcgtgat ggagtgctga cgggtgatat tgagatggct ttgttgcttg aaggaaatgc
      601 ccattaccga tgtgacttca gaactactta caaagctaag gagaagggtg tcaagttacc
      661 aggcgcccac tttgtggacc actgcattga gattttaagc catgacaaag attacaacaa
      721 ggttaagctg tatgagcatg ctgttgctca ttctggattg cctgacaatg ccagacgata
      781 agcggccgcg actctagatc ataatcagcc ataccacatt tgtagaggtt
//
