LOCUS       sequence-90598-e         829 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: N1 Cloning Vector, Excitation: 553, Emission: 574.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Merzlyak EM, Goedhart J, Shcherbo D, Bulina ME, Shcheglov AS, 
            Fradkov AF, Gaintzeva A, Lukyanov KA, Lukyanov S, Gadella TW, 
            Chudakov DM
  TITLE     Bright monomeric red fluorescent protein with an extended 
            fluorescence lifetime.
  JOURNAL   Nat Methods. 2007 Jul;4(7):555-7. Epub 2007 Jun 17.
  PUBMED    17572680
REFERENCE   2  (bases 1 to 829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods."; date: "2007-07"; volume: "4(7)"; pages: "555-7. Epub 2007
            Jun 17"
COMMENT     Sequence Label: TurboRFP-N1
FEATURES             Location/Qualifiers
     source          1..829
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    17..97
                     /label=MCS
                     /note="multiple cloning site"
     CDS             105..800
                     /codon_start=1
                     /product="red fluorescent protein from Entacmaea 
                     quadricolor"
                     /label=TurboRFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMKIKV
                     VEGGPLPFAFDILATSFMYGSKAFINHTQGIPDFFKQSFPEGFTWERITTYEDGGVLTA
                     TQDTSFQNGCIIYNVKINGVNFPSNGPVMQKKTRGWEANTEMLYPADGGLRGHSQMALK
                     LVGGGYLHCSFKTTYRSKKPAKNLKMPGFHFVDHRLERIKEADKETYVEQHEMAVAKYC
                     DLPSKLGHR"
ORIGIN
        1 gtgaaccgtc agatccgcta gcgctaccgg actcagatct cgagctcaag cttcgaattc
       61 tgcagtcgac ggtaccgcgg gcccgggatc caccggtcgc caccatgagc gagctgatca
      121 aggagaacat gcacatgaag ctgtacatgg agggcaccgt gaacaaccac cacttcaagt
      181 gcacatccga gggcgaaggc aagccctacg agggcaccca gaccatgaag atcaaggtgg
      241 tcgagggcgg ccctctcccc ttcgccttcg acatcctggc taccagcttc atgtacggca
      301 gcaaagcctt catcaaccac acccagggca tccccgactt ctttaagcag tccttccctg
      361 agggcttcac atgggagaga atcaccacat acgaagacgg gggcgtgctg accgctaccc
      421 aggacaccag cttccagaac ggctgcatca tctacaacgt caagatcaac ggggtgaact
      481 tcccatccaa cggccctgtg atgcagaaga aaacacgcgg ctgggaggcc aacaccgaga
      541 tgctgtaccc cgctgacggc ggcctgagag gccacagcca gatggccctg aagctcgtgg
      601 gcgggggcta cctgcactgc tccttcaaga ccacatacag atccaagaaa cccgctaaga
      661 acctcaagat gcccggcttc cacttcgtgg accacagact ggaaagaatc aaggaggccg
      721 acaaagagac ctacgtcgag cagcacgaga tggctgtggc caagtactgc gacctcccta
      781 gcaaactggg gcacagatga tgcggccgcg actctagatc ataatcagc
//
