LOCUS       sequence-90611-e         841 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Binder A, Lambert J, Morbitzer R, Popp C, Ott T, Lahaye T, Parniske 
            M
  TITLE     A modular plasmid assembly kit for multigene expression, gene 
            silencing and silencing rescue in plants.
  JOURNAL   PLoS One. 2014 Feb 13;9(2):e88218. doi: 
            10.1371/journal.pone.0088218. eCollection 2014.
  PUBMED    24551083
REFERENCE   2  (bases 1 to 841)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-02-13"; volume: "9(2):e88218. doi"; pages: " 
            10.1371/journal.pone.0088218. eCollection 2014"
COMMENT     Sequence Label: LIIIalpha F C-D
FEATURES             Location/Qualifiers
     source          1..841
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(196..201,202..219,220..226)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(346..365)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 tttttaatgt actggggtgg attacgggtc tcacacctac gttgtcttca aagctgaacg
       61 aaaaacgtaa aatgatataa atatcaatat attaaattag attttgcata aaaaacagac
      121 tacataatac tgtaaaacac aacatatcca gtcactatgg cggcccctcg aggggccgca
      181 ttaggcaccc caggctttac actttatgct tccggctcgt ataatgtgtg gattttgagt
      241 taggatccgt cgagattttc aggagctaag gaagctaaaa tggagaaaaa aatcactgga
      301 tataccaccg ttgatatatc ccaatggcat cgtaaagaac attttgaggc atttcagtca
      361 gttgctcaat gtacctataa ccagaccgtt cagctggata ttacggcctt tttaaagacc
      421 gtaaagaaaa ataagcacaa gttttatccg gcctttattc acattcttgc ccgcctgatg
      481 aatgctcatc cggaattccg tatggcaatg aaagacggtg agctggtgat atgggatagt
      541 gttcaccctt gttacaccgt tttccatgag caaactgaaa cgttttcatc gctctggagt
      601 gaataccacg acgatttccg gcagtttcta cacatatatt cgcaagatgt ggcgtgttac
      661 ggtgaaaacc tggcctattt ccctaaaggg tttattgaga atatgttttt cgtgtcagcc
      721 aatccctggg tgagtttcac cagttttgat ttaaacgtgg ccaatatgga caacttcttc
      781 gcccccgttt tcaccatggg caaatattat acgcaaggcg acaaggtgct gatgccgctg
      841 g
//
