LOCUS       sequence-90618-e         857 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 857)
  AUTHORS   Binder A, Lambert J, Morbitzer R, Popp C, Ott T, Lahaye T, Parniske 
            M
  TITLE     A modular plasmid assembly kit for multigene expression, gene 
            silencing and silencing rescue in plants.
  JOURNAL   PLoS One. 2014 Feb 13;9(2):e88218. doi: 
            10.1371/journal.pone.0088218. eCollection 2014.
  PUBMED    24551083
REFERENCE   2  (bases 1 to 857)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-02-13"; volume: "9(2):e88218. doi"; pages: " 
            10.1371/journal.pone.0088218. eCollection 2014"
COMMENT     Sequence Label: LIIIbeta fin
FEATURES             Location/Qualifiers
     source          1..857
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(145..150,151..168,169..175)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(295..314)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 agctgaacga gaaacgtaaa atgatataaa tatcaatata ttaaattaga ttttgcataa
       61 aaaacagact acataatact gtaaaacaca acatatccag tcactatggc ggcccctcga
      121 ggggccgcat taggcacccc aggctttaca ctttatgctt ccggctcgta taatgtgtgg
      181 attttgagtt aggatccgtc gagattttca ggagctaagg aagctaaaat ggagaaaaaa
      241 atcactggat ataccaccgt tgatatatcc caatggcatc gtaaagaaca ttttgaggca
      301 tttcagtcag ttgctcaatg tacctataac cagaccgttc agctggatat tacggccttt
      361 ttaaagaccg taaagaaaaa taagcacaag ttttatccgg cctttattca cattcttgcc
      421 cgcctgatga atgctcatcc ggaattccgt atggcaatga aagacggtga gctggtgata
      481 tgggatagtg ttcacccttg ttacaccgtt ttccatgagc aaactgaaac gttttcatcg
      541 ctctggagtg aataccacga cgatttccgg cagtttctac acatatattc gcaagatgtg
      601 gcgtgttacg gtgaaaacct ggcctatttc cctaaagggt ttattgagaa tatgtttttc
      661 gtgtcagcca atccctgggt gagtttcacc agttttgatt taaacgtggc caatatggac
      721 aacttcttcg cccccgtttt caccatgggc aaatattata cgcaaggcga caaggtgctg
      781 atgccgctgg cgattcaggt tcatcatgcc gtttgtgatg gcttccatgt cggcagaatg
      841 cttaatgaat tacaaca
//
