LOCUS       sequence-90620-e         910 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Binder A, Lambert J, Morbitzer R, Popp C, Ott T, Lahaye T, Parniske 
            M
  TITLE     A modular plasmid assembly kit for multigene expression, gene 
            silencing and silencing rescue in plants.
  JOURNAL   PLoS One. 2014 Feb 13;9(2):e88218. doi: 
            10.1371/journal.pone.0088218. eCollection 2014.
  PUBMED    24551083
REFERENCE   2  (bases 1 to 910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-02-13"; volume: "9(2):e88218. doi"; pages: " 
            10.1371/journal.pone.0088218. eCollection 2014"
COMMENT     Sequence Label: LIIIbeta F E-F
FEATURES             Location/Qualifiers
     source          1..910
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(163..168,169..186,187..193)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             247..906
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(313..332)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 aatctacgtt gtcttcaaag ctgaacgaga aacgtaaaat gatataaata tcaatatatt
       61 aaattagatt ttgcataaaa aacagactac ataatactgt aaaacacaac atatccagtc
      121 actatggcgg cccctcgagg ggccgcatta ggcaccccag gctttacact ttatgcttcc
      181 ggctcgtata atgtgtggat tttgagttag gatccgtcga gattttcagg agctaaggaa
      241 gctaaaatgg agaaaaaaat cactggatat accaccgttg atatatccca atggcatcgt
      301 aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta cctataacca gaccgttcag
      361 ctggatatta cggccttttt aaagaccgta aagaaaaata agcacaagtt ttatccggcc
      421 tttattcaca ttcttgcccg cctgatgaat gctcatccgg aattccgtat ggcaatgaaa
      481 gacggtgagc tggtgatatg ggatagtgtt cacccttgtt acaccgtttt ccatgagcaa
      541 actgaaacgt tttcatcgct ctggagtgaa taccacgacg atttccggca gtttctacac
      601 atatattcgc aagatgtggc gtgttacggt gaaaacctgg cctatttccc taaagggttt
      661 attgagaata tgtttttcgt gtcagccaat ccctgggtga gtttcaccag ttttgattta
      721 aacgtggcca atatggacaa cttcttcgcc cccgttttca ccatgggcaa atattatacg
      781 caaggcgaca aggtgctgat gccgctggcg attcaggttc atcatgccgt ttgtgatggc
      841 ttccatgtcg gcagaatgct taatgaatta caacagtact gcgatgagtg gcagggcggg
      901 gcgtaatcta
//
