LOCUS       sequence-90621-e         728 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 728)
  AUTHORS   Binder A, Lambert J, Morbitzer R, Popp C, Ott T, Lahaye T, Parniske 
            M
  TITLE     A modular plasmid assembly kit for multigene expression, gene 
            silencing and silencing rescue in plants.
  JOURNAL   PLoS One. 2014 Feb 13;9(2):e88218. doi: 
            10.1371/journal.pone.0088218. eCollection 2014.
  PUBMED    24551083
REFERENCE   2  (bases 1 to 728)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-02-13"; volume: "9(2):e88218. doi"; pages: " 
            10.1371/journal.pone.0088218. eCollection 2014"
COMMENT     Sequence Label: LIIbeta F 5-6
FEATURES             Location/Qualifiers
     source          1..728
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(164..169,170..187,188..194)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(314..333)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 aatgaggcgg tgagaccaaa gctgaacgag aaacgtaaaa tgatataaat atcaatatat
       61 taaattagat tttgcataaa aaacagacta cataatactg taaaacacaa catatccagt
      121 cactatggcg gcccctcgag gggccgcatt aggcacccca ggctttacac tttatgcttc
      181 cggctcgtat aatgtgtgga ttttgagtta ggatccgtcg agattttcag gagctaagga
      241 agctaaaatg gagaaaaaaa tcactggata taccaccgtt gatatatccc aatggcatcg
      301 taaagaacat tttgaggcat ttcagtcagt tgctcaatgt acctataacc agaccgttca
      361 gctggatatt acggcctttt taaagaccgt aaagaaaaat aagcacaagt tttatccggc
      421 ctttattcac attcttgccc gcctgatgaa tgctcatccg gaattccgta tggcaatgaa
      481 agacggtgag ctggtgatat gggatagtgt tcacccttgt tacaccgttt tccatgagca
      541 aactgaaacg ttttcatcgc tctggagtga ataccacgac gatttccggc agtttctaca
      601 catatattcg caagatgtgg cgtgttacgg tgaaaacctg gcctatttcc ctaaagggtt
      661 tattgagaat atgtttttcg tgtcagccaa tccctgggtg agtttcacca gttttgattt
      721 aaacgtgg
//
