LOCUS       sequence-90624-e         879 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 879)
  AUTHORS   Binder A, Lambert J, Morbitzer R, Popp C, Ott T, Lahaye T, Parniske 
            M
  TITLE     A modular plasmid assembly kit for multigene expression, gene 
            silencing and silencing rescue in plants.
  JOURNAL   PLoS One. 2014 Feb 13;9(2):e88218. doi: 
            10.1371/journal.pone.0088218. eCollection 2014.
  PUBMED    24551083
REFERENCE   2  (bases 1 to 879)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-02-13"; volume: "9(2):e88218. doi"; pages: " 
            10.1371/journal.pone.0088218. eCollection 2014"
COMMENT     Sequence Label: LIIbeta R 3-4
FEATURES             Location/Qualifiers
     source          1..879
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    18..42
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             complement(128..433)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(230..249)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 acgctctttt ctcttaggtt tacccgccaa tatatcctgt caaacactga tagtttaaac
       61 cagagaagac aagacagcgg tgagaccgtc gacctgcaga ctggctgtgt ataagggagc
      121 ctgacattta tattccccag aacatcaggt taatggcgtt tttgatgtca ttttcgcggt
      181 ggctgagatc agccacttct tccccgataa cggaaaccgg cacactggcc atatcggtgg
      241 tcatcatgcg ccagctttca tccccgatat gcaccaccgg gtaaagttca cgggggactt
      301 tatctgacag cagacgtgca ctggccaggg ggatcaccat ccgtcgcccg ggcgtgtcaa
      361 taatatcact ctgtacatcc acaaacagac gataacggct ctctctttta taggtgtaaa
      421 ccttaaactg catttcacca gcccctgttc tcgtcagcaa aagagccgtt catttcaata
      481 aaccgggcga cctcagccat cccttcctga ttttccgctt tccagcgttc ggcacgcaga
      541 cgacgggctt cattctgcat ggttgtgctt accagaccgg agatattgac atcatatatg
      601 ccttgagcaa ctgatagctg tcgctgtcaa ctgtcactgt aatacgctgc ttcatagcat
      661 acctcttttt gacatacttc gggtatacat atcagtatat attcttatac cgcaaaaatc
      721 agcgcgcaaa tacgcatact gttatctggc ttttagtaag ccggatcctc tagattacgc
      781 cccgccctgc cactcatcgc agtactgttg taattcatta agcattctgc cgacatggaa
      841 gcccatcaca aacggcatga tgaacctgaa tcgccagcg
//
