LOCUS       sequence-90625-e         557 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 557)
  AUTHORS   Binder A, Lambert J, Morbitzer R, Popp C, Ott T, Lahaye T, Parniske 
            M
  TITLE     A modular plasmid assembly kit for multigene expression, gene 
            silencing and silencing rescue in plants.
  JOURNAL   PLoS One. 2014 Feb 13;9(2):e88218. doi: 
            10.1371/journal.pone.0088218. eCollection 2014.
  PUBMED    24551083
REFERENCE   2  (bases 1 to 557)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-02-13"; volume: "9(2):e88218. doi"; pages: " 
            10.1371/journal.pone.0088218. eCollection 2014"
COMMENT     Sequence Label: LIIalpha R 5-6
FEATURES             Location/Qualifiers
     source          1..557
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    1..25
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             complement(111..416)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(213..232)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 gtttacccgc caatatatcc tgtcaaacac tgatagttta aaccagagaa gacaacagag
       61 cggtgagacc gtcgacctgc agactggctg tgtataaggg agcctgacat ttatattccc
      121 cagaacatca ggttaatggc gtttttgatg tcattttcgc ggtggctgag atcagccact
      181 tcttccccga taacggaaac cggcacactg gccatatcgg tggtcatcat gcgccagctt
      241 tcatccccga tatgcaccac cgggtaaagt tcacggggga ctttatctga cagcagacgt
      301 gcactggcca gggggatcac catccgtcgc ccgggcgtgt caataatatc actctgtaca
      361 tccacaaaca gacgataacg gctctctctt ttataggtgt aaaccttaaa ctgcatttca
      421 ccagcccctg ttctcgtcag caaaagagcc gttcatttca ataaaccggg cgacctcagc
      481 catcccttcc tgattttccg ctttccagcg ttcggcacgc agacgacggg cttcattctg
      541 catggttgtg cttacca
//
