LOCUS       sequence-90627-e         826 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Binder A, Lambert J, Morbitzer R, Popp C, Ott T, Lahaye T, Parniske 
            M
  TITLE     A modular plasmid assembly kit for multigene expression, gene 
            silencing and silencing rescue in plants.
  JOURNAL   PLoS One. 2014 Feb 13;9(2):e88218. doi: 
            10.1371/journal.pone.0088218. eCollection 2014.
  PUBMED    24551083
REFERENCE   2  (bases 1 to 826)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-02-13"; volume: "9(2):e88218. doi"; pages: " 
            10.1371/journal.pone.0088218. eCollection 2014"
COMMENT     Sequence Label: LIIalpha F 4-5
FEATURES             Location/Qualifiers
     source          1..826
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    19..43
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             complement(129..434)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(231..250)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 aacgctcttt tctcttaggt ttacccgcca atatatcctg tcaaacactg atagttcaaa
       61 ccagagaaga caacagagcg gtgagaccgt cgacctgcag actggctgtg tataagggag
      121 cctgacattt atattcccca gaacatcagg ttaatggcgt ttttgatgtc attttcgcgg
      181 tggctgagat cagccacttc ttccccgata acggaaaccg gcacactggc catatcggtg
      241 gtcatcatgc gccagctttc atccccgata tgcaccaccg ggtaaagttc acgggggact
      301 ttatctgaca gcagacgtgc actggccagg gggatcacca tccgtcgccc gggcgtgtca
      361 ataatatcac tctgtacatc cacaaacaga cgataacggc tctctctttt ataggtgtaa
      421 accttaaact gcatttcacc agcccctgtt ctcgtcagca aaagagccgt tcatttcaat
      481 aaaccgggcg acctcagcca tcccttcctg attttccgct ttccagcgtt cggcacgcag
      541 acgacgggct tcattctgca tggttgtgct taccagaccg gagatattga catcatatat
      601 gccttgagca actgatagct gtcgctgtca actgtcactg taatacgctg cttcatagca
      661 tacctctttt tgacatactt cgggtataca tatcagtata tattcttata ccgcaaaaat
      721 cagcgcgcaa aatacgcata ctgttatctg gcttttagta agccggatcc tctagattac
      781 gcccccgccc tgccactcat ccgcagtact gttggaaatt cattaa
//
