LOCUS       sequence-90635-e         471 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Baculovirus expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 471)
  TITLE     SGC_OxfordQ3Q4_2013
REFERENCE   2  (bases 1 to 471)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: UTYC-c613
FEATURES             Location/Qualifiers
     source          1..471
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(29..51)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    40..56
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(64..70,71..88,89..94))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    109..130
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 ttggcgtaat catggtcata gcgtgtttcc tgtgtgaaat tgttatccgc tcacaattcc
       61 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
      121 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
      181 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
      241 cgcttccgcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
      301 tcactcaaag gcggtaatac ggttatccac ggaatcaggg gataacgcag gaaagaacat
      361 gtgagcaaaa ggccagcgaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
      421 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc a
//
