LOCUS       sequence-90660-e        2630 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses 3x Flag-tagged LGP2 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2630)
  AUTHORS   Bamming D, Horvath CM
  TITLE     Regulation of signal transduction by enzymatically inactive 
            antiviral RNA helicase proteins MDA5, RIG-I, and LGP2.
  JOURNAL   J Biol Chem. 2009 Apr 10;284(15):9700-12. doi: 
            10.1074/jbc.M807365200. Epub 2009 Feb 11.
  PUBMED    19211564
REFERENCE   2  (bases 1 to 2630)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M807365200"; journalName: "J Biol Chem"; date: 
            "2009-04-10- 10"; volume: "284"; issue: "15"; pages: "9700-12"
COMMENT     Sequence Label: CMV-10-3xFLAG-LGP2
FEATURES             Location/Qualifiers
     source          1..2630
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..69
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             complement(2568..2579)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 atggactaca aagaccatga cggtgattat aaagatcatg acatcgatta caaggatgac
       61 gatgacaagc ttgcggccgc gatggagctt cggtcctacc aatgggaggt gatcatgcct
      121 gccctggagg gcaagaatat catcatctgg ctgcccacgg gtgccgggaa gacccgggcg
      181 gctgcttatg tggccaagcg gcacctagag actgtggatg gagccaaggt ggttgtattg
      241 gtcaacaggg tgcacctggt gacccagcat ggtgaagagt tcaggcgcat gctggatgga
      301 cgctggaccg tgacaaccct gagtggggac atgggaccac gtgctggctt tggccacctg
      361 gcccggtgcc atgacctgct catctgcaca gcagagcttc tgcagatggc actgaccagc
      421 cccgaggagg aggagcacgt ggagctcact gtcttctccc tgatcgtggt ggatgagtgc
      481 caccacacgc acaaggacac cgtctacaac gtcatcatga gccagtacct agaacttaaa
      541 ctccagaggg cacagccgct accccaggtg ctgggtctca cagcctcccc aggcactggc
      601 ggggcctcca aactcgatgg ggccatcaac cacgtcctgc agctctgtgc caacttggac
      661 acgtggtgca tcatgtcacc ccagaactgc tgcccccagc tgcaggagca cagccaacag
      721 ccttgcaaac agtacaacct ctgccacagg cgcagccagg atccgtttgg ggacttgctg
      781 aagaagctca tggaccaaat ccatgaccac ctggagatgc ctgagttgag ccggaaattt
      841 gggacgcaaa tgtatgagca gcaggtggtg aagctgagtg aggctgcggc tttggctggg
      901 cttcaggagc aacgggtgta tgcgcttcac ctgaggcgct acaatgacgc gctgctcatc
      961 catgacaccg tccgcgccgt ggatgccttg gctgcgctgc aggatttcta tcacagggag
     1021 cacgtcacta aaacccagat cctgtgtgcc gagcgccggc tgctggccct gttcgatgac
     1081 cgcaagaatg agctggccca cttggcaact catggcccag agaatccaaa actggagatg
     1141 ctggaaaaga tcctgcaaag gcagttcagt agctctaaca gccctcgggg tatcatcttc
     1201 acccgcaccc gccaaagcgc acactccctc ctgctctggc tccagcagca gcagggcctg
     1261 cagactgtgg acatccgggc ccagctactg attggggctg ggaacagcag ccagagcacc
     1321 cacatgaccc agagggacca gcaagaagtg atccagaagt tccaagatgg aaccctgaac
     1381 cttctggtgg ccacgagtgt ggcggaggag gggctggaca tcccacattg caatgtggtg
     1441 gtgcgttatg ggctcttgac caatgaaatc tccatggtcc aggccagggg ccgtgcccgg
     1501 gccgatcaga gtgtatacgc gtttgtagca actgaaggta gccgggagct gaagcgggag
     1561 ctgatcaacg aggcgctgga gacgctgatg gagcaggcag tggctgctgt gcagaaaatg
     1621 gaccaggccg agtaccaggc caagatccgg gatctgcagc aggcagcctt gaccaagcgg
     1681 gcggcccagg cagcccagcg ggagaaccag cggcagcagt tcccagtgga gcacgtgcag
     1741 ctactctgca tcaactgcat ggtggctgtg ggccatggca gcgacctgcg gaaggtggag
     1801 ggcacccacc atgtcaatgt gaaccccaac ttctcgaact actataatgt ctccagggat
     1861 cctgtggtca tcaacaaagt cttcaaggac tggaagcctg ggggtgtcat cagctgcagg
     1921 aactgtgggg aggtctgggg tctgcagatg atctacaagt cagtgaagct gccagtgctc
     1981 aaagtccgca gcatgctgct ggagacccct caggggcgga tccaggccaa aaagtggtcc
     2041 cgcgtgccct tctccgtgcc tgactttgac ttcctgcagc attgtgccga gaacttgtcg
     2101 gacctctccc tggactgatc tagaggatcc cgggtggcat ccctgtgacc cctccccagt
     2161 gcctctcctg gccctggaag ttgccactcc agtgcccacc agccttgtcc taataaaatt
     2221 aagttgcatc attttgtctg actaggtgtc cttctataat attatgagtg aaaaggggtt
     2281 tctaacggac cagggggcaa tttgggaaac aacctgtagg gcctgcgggg tctatttggg
     2341 aaccaagctg gagtgcagtg ccacaatctt gctcactgca atctccgcct cctggggttc
     2401 aagcgattct ctgctcagct cccgagttgt ggattccagc atgcatgaca gctcagctat
     2461 tttgtttttt tggtagagac gggattcacc atatgacaag ctggcttcac tctatctcag
     2521 gtgatctaca cttgactcta atgctgcata cgcctgacac tgctcttccg tccttcgatt
     2581 tgaatagcta tcagcagaga gcttcagaac agcagctatc ctgccgtgtc
//
