LOCUS       sequence-90665-e        2176 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pDONR P4-P1r carrying the STE2 promoter Citrine fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2176)
  TITLE     Fluorescent X-QTL
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2176)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: P4-P1r Ste2pCitrine
FEATURES             Location/Qualifiers
     source          1..2176
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    6..26
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             1434..2150
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSRLSKDPNEKRDHMVLLE
                     FVTAAGITHGIDELYK"
     protein_bind    2150..2174
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ggggacaact ttgtatagaa aagttggacc caatagcgaa aaggggctca ttttcgtcaa
       61 atttgaatag attatagaat ttcatgtttg attcctttat caatgaagag attactctca
      121 attgctcaaa tgaaaaatct agtgagttca tcatgaaatc cctcagatat aatagggtaa
      181 atgcttcgat gcaataaaac agcctttcca tatcaatttc ttcttgcaat gagagatttt
      241 ctagttccag tccgttctct tttgtgacat tccaactaaa acacatgatg aaaacagaca
      301 caatatcatg ataaccttgg taatagttca acgttgggta ctttctcaat acccgagtaa
      361 ttaactccac cagtagtttt ctcaattgca gtttcaagtt tttgtcgaca attccaccaa
      421 aagacctttc tgcatccaat atcacctgac cttcatcttt atgtttttcc actggtgtaa
      481 tgtactctgt tgcatcatca ataagcaact gggacgccaa taattcatac caacaatctt
      541 ttcttaatga gttgtttaca aaccccagag gccctaaacc tatgtacctc aaagctcgga
      601 catctttttt ctctaataac tttgatataa cttttccttt ttgttcttct gcctttccac
      661 tgtcacaacg agaaagtgat tccagatatg cgttataacc tccccttgtc aacgcgtctt
      721 tgtcgtggct actctgatta gtatgaaata atgaccttaa tggcatcgta ttttgttaat
      781 tggcttgttc tatgtcattt gctaaatttt ctctttattt ctttactttg ccaattcaag
      841 ataatcttcc cttcccagag agaaaaaagg aaaaatttag ctatgaaacc tcaataagct
      901 ttttaataca ccaaagattc aagataagag catagaacga actgtagaat agtccggata
      961 tgttatccaa tgcctgccaa aatgcattgt cacacgctgt agtgctcgaa taggtgttgc
     1021 aatccgtcaa tatacgtctt gctctgtggg taaatgtctc gtgcattaag acaggctagt
     1081 ataaacgaga agaagtatcc tgctttgcaa tgaaacaata gtatccgcta agaatttaag
     1141 caggccaacg tccatactgc ttaggacctg tgcctggcaa gtcgcagatt gaagtttttt
     1201 caaccatgta aatttcctaa ttgggtaagt acatgatgaa acacatatga agaaaaaagc
     1261 tttcctacat attcaagatt tttttctgtg ggtggaatac tatttaagga gtgctattag
     1321 tatcttattt gacttcaaag caatacgata ccttttcttt tcacctgctc tggctataat
     1381 tataattggt tacttaaaaa tgcaccgtta agaaccatat ccaagaatca aaaatgtcta
     1441 aaggtgaaga attattcact ggtgttgtcc caattttggt tgaattagat ggtgatgtta
     1501 atggtcacaa attttctgtc tccggtgaag gtgaaggtga tgctacttac ggtaaattga
     1561 ccttaaaatt tatttgtact actggtaaat tgccagttcc atggccaacc ttagtcacta
     1621 ctttaggtta tggtttgatg tgttttgcta gatacccaga tcatatgaaa caacatgact
     1681 ttttcaagtc tgccatgcca gaaggttatg ttcaagaaag aactattttt ttcaaagatg
     1741 acggtaacta caagaccaga gctgaagtca agtttgaagg tgatacctta gttaatagaa
     1801 tcgaattaaa aggtattgat tttaaagaag atggtaacat tttaggtcac aaattggaat
     1861 acaactataa ctctcacaat gtttacatca tggctgacaa acaaaagaat ggtatcaaag
     1921 ttaacttcaa aattagacac aacattgaag atggttctgt tcaattagct gaccattatc
     1981 aacaaaatac tccaattggt gatggtccag tcttgttacc agacaaccat tacttatcct
     2041 atcaatctag attatccaaa gatccaaacg aaaagagaga ccacatggtc ttgttagaat
     2101 ttgttactgc tgctggtatt acccatggta ttgatgaatt gtacaaataa caagtttgta
     2161 caaaaaagca gtcccc
//
