LOCUS       sequence-90675-e         530 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Reporter construct containing a 742 bp promoter region of the Ihh 
            gene in front of a luciferase gene for in vitro DNA transfection 
            assays.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 530)
  AUTHORS   Wang W, Lian N, Li L, Moss HE, Wang W, Perrien DS, Elefteriou F, 
            Yang X
  TITLE     Atf4 regulates chondrocyte proliferation and differentiation during 
            endochondral ossification by activating Ihh transcription.
  JOURNAL   Development. 2009 Dec;136(24):4143-53. doi: 10.1242/dev.043281. Epub
            2009 Nov 11.
  PUBMED    19906842
REFERENCE   2  (bases 1 to 530)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1242/dev.043281"; journalName: "Development"; date: "2009-12"; 
            volume: "136"; issue: "24"; pages: "4143-53"
COMMENT     Sequence Label: pIhh742bp-Luc
FEATURES             Location/Qualifiers
     source          1..530
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     19..41
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     33..50
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     34..50
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     57..76
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        57..75
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    257..391
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(294..313)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     348..367
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt gaattgtaat
       61 acgactcact atagggcgaa ttgggcccga cgtcgcatgc tcctctagag cttgcagatc
      121 tgcgactcta gaggatctgc gactctagag gatcataatc agccatacca catttgtaga
      181 ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa
      241 tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag
      301 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
      361 actcatcaat gtatcttatc atgtctggat ctgcgactct agaggatcat aatcagccat
      421 accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg
      481 aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta
//
