LOCUS       sequence-90678-e         819 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  allows Cre-mediated expression of transgene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Nakamura T, Gulick J, Pratt R, Robbins J
  TITLE     Noonan syndrome is associated with enhanced pERK activity, the 
            repression of which can prevent craniofacial malformations.
  JOURNAL   Proc Natl Acad Sci U S A. 2009 Sep 8;106(36):15436-41. doi: 
            10.1073/pnas.0903302106. Epub 2009 Aug 24.
  PUBMED    19706403
REFERENCE   2  (bases 1 to 819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.0903302106"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2009-09-8- 8"; volume: "106"; issue: "36"; pages: "15436-41"
COMMENT     Sequence Label: CAG-lox-CAT-lox-unique Bam cloning site-hGH po...
FEATURES             Location/Qualifiers
     source          1..819
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     8..28
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10..28
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     464..483
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     protein_bind    700..733
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(779..798)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 ccagcgcgca attaaccctc actaaaggga acaaaagctg gagctccacc gcggtggcgg
       61 ccgcaacagg catctactga gtggacccaa cgcatgagag gacagtgcca agcaagcaac
      121 tcaaatgtcc caccggttgg gcatggccag gtagcctatg ctgtgtctgg acgtcctcct
      181 gctggtatag ttattttaaa atcagaagga cagggaaggg agcagtggtt cacgcctgta
      241 atcccagcaa tttgggaggc caaggtgggt agatcacctg agattaggag ttggagacca
      301 gcctggccaa tatggtgaaa ccccgtctct accaaaaaaa caaaaattag ctgagcctgg
      361 tcatgcatgc ctggaatccc aacaactcgg gaggctgagg caggagaatc gcttgaaccc
      421 aggaggcgga gattgcagtg agccaagatt gtgccactgc actccagctt ggttcccaat
      481 agaccccgca ggccctacag gttgtcttcc caacttgccc cttgctccat accacccccc
      541 tccaccccat aatattatag aaggacacct agtcagacaa aatgatgcaa cttaatttta
      601 ttaggacaag gctggtgggc actggagtgg caacttccag ggccaggaga ggcactgggg
      661 aggggtcaca gggatgccac cggatccgga acccttaata taacttcgta taatgtatgc
      721 tatacgaagt tattaggtcc ctcgaccggg gatcgatcca gacatgataa gatacattga
      781 tgagtttgga caaaccacaa ctagaatgca gtgaaaaaa
//
