LOCUS       sequence-90699-e         842 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin, Excitation: 399, Emission: 456.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 842)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: mTagBFP-Lifeact-7
FEATURES             Location/Qualifiers
     source          1..842
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      34..43
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             40..90
                     /codon_start=1
                     /product="peptide that binds filamentous actin (Riedl et 
                     al., 2008)"
                     /label=Lifeact
                     /note="first 17 amino acids of budding yeast Abp140"
                     /translation="MGVADLIKKFESISKEE"
     CDS             112..813
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
ORIGIN
        1 gagctggttt agtgaaccgt cagatccgct agcgccacca tgggcgtggc cgacttgatc
       61 aagaagttcg agtccatctc caaggaggag ggggatccac cggtcgccac catgagcgag
      121 ctgattaagg agaacatgca catgaagctg tacatggagg gcaccgtgga caaccatcac
      181 ttcaagtgca catccgaggg cgaaggcaag ccctacgagg gcacccagac catgagaatc
      241 aaggtggtcg agggcggccc tctccccttc gccttcgaca tcctggctac tagcttcctc
      301 tacggcagca agaccttcat caaccacacc cagggcatcc ccgacttctt caagcagtcc
      361 ttccctgagg gcttcacatg ggagagagtc accacatacg aagacggggg cgtgctgacc
      421 gctacccagg acaccagcct ccaggacggc tgcctcatct acaacgtcaa gatcagaggg
      481 gtgaacttca catccaacgg ccctgtgatg cagaagaaaa cactcggctg ggaggccttc
      541 accgagacgc tgtaccccgc tgacggcggc ctggaaggca gaaacgacat ggccctgaag
      601 ctcgtgggcg ggagccatct gatcgcaaac atcaagacca catatagatc caagaaaccc
      661 gctaagaacc tcaagatgcc tggcgtctac tatgtggact acagactgga aagaatcaag
      721 gaggccaaca acgagaccta cgtcgagcag cacgaggtgg cagtggccag atactgcgac
      781 ctccctagca aactggggca caagcttaat taaagcggcc gcgactctag atcataatca
      841 gc
//
