LOCUS       sequence-90700-e         817 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: C1 Cloning Vector, Excitation: 462, Emission: 492.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 817)
  AUTHORS   Ai HW, Olenych SG, Wong P, Davidson MW, Campbell RE
  TITLE     Hue-shifted monomeric variants of Clavularia cyan fluorescent 
            protein: identification of the molecular determinants of color and 
            applications in fluorescence imaging.
  JOURNAL   BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/1741-7007-6-13.
  PUBMED    18325109
REFERENCE   2  (bases 1 to 817)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Biol.";
            date: "2008-03-6"; volume: "6:13. doi"; pages: " 
            10.1186/1741-7007-6-13"
COMMENT     Sequence Label: mTFP1-C1
FEATURES             Location/Qualifiers
     source          1..817
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      48..57
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             54..761
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
                     GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
                     VARNSTDGMDELYK"
ORIGIN
        1 agcagagctg gtttagtgaa ccgtcagatc cgctagcgct accggtcgcc accatggtga
       61 gcaagggcga ggagaccaca atgggcgtaa tcaagcccga catgaagatc aagctgaaga
      121 tggagggcaa cgtgaatggc cacgccttcg tgatcgaggg cgagggcgag ggcaagccct
      181 acgacggcac caacaccatc aacctggagg tgaaggaggg agcccccctg cccttctcct
      241 acgacattct gaccaccgcg ttcgcctacg gcaacagggc cttcaccaag taccccgacg
      301 acatccccaa ctacttcaag cagtccttcc ccgagggcta ctcttgggag cgcaccatga
      361 ccttcgagga caagggcatc gtgaaggtga agtccgacat ctccatggag gaggactcct
      421 tcatctacga gatacacctc aagggcgaga acttcccccc caacggcccc gtgatgcaga
      481 agaagaccac cggctgggac gcctccaccg agaggatgta cgtgcgcgac ggcgtgctga
      541 agggcgacgt caagcacaag ctgctgctgg agggcggcgg ccaccaccgc gttgacttca
      601 agaccatcta cagggccaag aaggcggtga agctgcccga ctatcacttt gtggaccacc
      661 gcatcgagat cctgaaccac gacaaggact acaacaaggt gaccgtttac gagagcgccg
      721 tggcccgcaa ctccaccgac ggcatggacg agctgtacaa gtccggactc agatctcgag
      781 ctcaagcttc gaattctgca gtcgacggta ccgcggg
//
