LOCUS       sequence-90711-e         825 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: C1 Cloning Vector, Excitation: 505 / 569, Emission: 
            516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Paez-Segala MG, Sun MG, Shtengel G, Viswanathan S, Baird MA, Macklin
            JJ, Patel R, Allen JR, Howe ES, Piszczek G, Hess HF, Davidson MW, 
            Wang Y, Looger LL
  TITLE     Fixation-resistant photoactivatable fluorescent proteins for CLEM.
  JOURNAL   Nat Methods. 2015 Jan 12. doi: 10.1038/nmeth.3225.
  PUBMED    25581799
REFERENCE   2  (bases 1 to 825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Jan 12. doi: 10.1038/nmeth.3225."
COMMENT     Sequence Label: mEos4b-C1
FEATURES             Location/Qualifiers
     source          1..825
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      33..42
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             39..719
                     /codon_start=1
                     /product="fixation-resistant truly monomeric green-to-red 
                     photoswitchable fluorescent protein (Paez-Segala et al., 
                     2015)"
                     /label=mEos4b
                     /note="derived from mEos2"
                     /translation="MVSAIKPDMRIKLRMEGNVNGHHFVIDGDGTGKPYEGKQTMDLEV
                     KEGGPLPFAFDILTTAFHYGNRVFVKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNA
                     RNDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIEMALL
                     LEGNAHYRCDFRTTYKAKEKGVKLPGAHFVDHAIEILSHDKDYNKVKLYEHAVAHSGLP
                     DNARR"
     misc_feature    720..785
                     /label=MCS
                     /note="multiple cloning site"
ORIGIN
        1 gtgaaccgtc agatccgcta gcgctaccgg tcgccaccat ggtgagtgcg attaagccag
       61 acatgaggat caaactccgt atggaaggca acgtaaacgg gcaccacttt gtgatcgacg
      121 gagatggtac aggcaagcct tatgagggaa aacagaccat ggatcttgaa gtcaaagagg
      181 gcggacctct gccttttgcc tttgatatcc tgaccactgc attccattac ggcaacaggg
      241 tattcgtgaa atatccagac aacatacaag actattttaa gcagtcgttt cctaaggggt
      301 attcgtggga acgaagcttg actttcgaag acgggggcat ttgcaatgcc agaaacgaca
      361 taacaatgga aggggacact ttctataata aagttcgatt ttatggtacc aactttcccg
      421 ccaatggtcc agttatgcag aagaagacgc tgaaatggga gccctccact gagaaaatgt
      481 atgtgcgtga tggagtgctg acgggtgata ttgagatggc tttgttgctt gaaggaaatg
      541 cccattaccg atgtgacttc agaactactt acaaagctaa ggagaagggt gtcaagttac
      601 caggcgccca ctttgtggac cacgccattg agattttaag ccatgacaaa gattacaaca
      661 aggttaagct gtatgagcat gctgttgctc attctggatt gcctgacaat gccagacgat
      721 ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc gcgggcccgg
      781 gatccaccgg atctagataa ctgatcataa tcagccatac cacat
//
