LOCUS       sequence-90712-e         793 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin, Excitation: 514, Emission: 527.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 793)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 793)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: mTopaz-Lifeact-7
FEATURES             Location/Qualifiers
     source          1..793
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      33..42
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             39..89
                     /codon_start=1
                     /product="peptide that binds filamentous actin (Riedl et 
                     al., 2008)"
                     /label=Lifeact
                     /note="first 17 amino acids of budding yeast Abp140"
                     /translation="MGVADLIKKFESISKEE"
     regulatory      105..114
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(111..113,114..116,117..629)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMRQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     complement(156..177)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(417..436)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     764..785
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 agctggttta gtgaaccgtc agatccgcta gcgccaccat gggcgtggcc gacttgatca
       61 agaagttcga gtccatctcc aaggaggagg gggatccacc ggtcgccacc atggtgagca
      121 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
      181 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
      241 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
      301 ccttcggcta cggcgtgcag tgcttcgccc gctaccccga ccacatgcgc cagcacgact
      361 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
      421 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
      481 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
      541 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg
      601 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc
      661 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagct
      721 accagtccaa gctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
      781 tcgtgaccgc cgc
//
