LOCUS       sequence-90719-e        9199 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  UAS vector to express gene of interest in Drosophila.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9199)
  AUTHORS   Han C, Jan LY, Jan YN
  TITLE     Enhancer-driven membrane markers for analysis of nonautonomous 
            mechanisms reveal neuron-glia interactions in Drosophila.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Jun 7;108(23):9673-8. doi: 
            10.1073/pnas.1106386108. Epub 2011 May 23.
  PUBMED    21606367
REFERENCE   2  (bases 1 to 9199)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1106386108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-06-7- 7"; volume: "108"; issue: "23"; pages: "9673-8"
COMMENT     Sequence Label: pACU
FEATURES             Location/Qualifiers
     source          1..9199
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(235..823)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(315..334)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(994..1785,1786..1854))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1617..1636
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1855..1959)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2027..2045
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2083..2105)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2205..2224
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     protein_bind    2434..2503
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     misc_feature    join(3046..3076,3077..3278)
                     /label=P element 3' end
                     /note="P element 3' end"
     protein_bind    3340..3434
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        3453..3691
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     3638..3659
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     intron          3828..3893
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4023..4043
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    4315..4449
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4352..4371)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4406..4425
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4476..4494)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(4954..5025,5420..5693,5768..6422,6484..6799,7020..7151,
                     7222..7836)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLLPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     misc_feature    join(8614..9168,9169..9199)
                     /label=P element 5' end
ORIGIN
        1 ggccagaccc acgtagtcca gcggcagatc ggcggcggag aagttaagcg tctccaggat
       61 gaccttgccc gaactggggc acgtggtgtt cgacgatgtg cagctaattt cgcccggctc
      121 cacgtccgcc cattggttaa tcagcagacc ctcgttggcg taacggaacc atgagaggta
      181 cgacaaccat ttgaggtata ctggcaccga gcccgagttc aagaagaagg cgtttttcca
      241 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
      301 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
      361 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
      421 gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
      481 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
      541 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
      601 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
      661 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
      721 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
      781 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
      841 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
      901 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
      961 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     1021 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     1081 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     1141 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     1201 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     1261 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     1321 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     1381 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     1441 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     1501 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     1561 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa
     1621 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     1681 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     1741 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     1801 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     1861 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     1921 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     1981 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
     2041 gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
     2101 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
     2161 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat
     2221 tgtactgaga gtgcaccata tcgacatgcc cgccgtgacc gtcgagaacc cgctgacgct
     2281 gccccgcgta tccgcacccg ccgacgccgt cgcacgtccc gtgctcaccg tgaccaccgc
     2341 gcccagcggt ttcgagggcg agggcttccc ggtgcgccgc gcgttcgccg ggatcaacta
     2401 ccgccacctc gacccgttca tcatgatgga ccagatgggt gaggtggagt acgcgcccgg
     2461 ggagcccaag ggcacgccct ggcacccgca ccgcggcttc gagaccgtga cctacatcgt
     2521 cgatatgcgg tgtgaaatac cgcaccgaat cgcgcggaac taacgacagt cgctccaagg
     2581 tcgtcgaaca aaaggtgaat gtgttgcgga gagcgggtgg gagacagcga aagagcaact
     2641 acgaaacgtg gtgtggtgga ggtgaattat gaagagggcg cgcgatttga aaagtatgta
     2701 tataaaaaat atatcccggt gttttatgta gcgataaacg agtttttgat gtaaggtatg
     2761 caggtgtgta agtcttttgg ttagaagaca aatccaaagt ctacttgtgg ggatgttcga
     2821 aggggaaata cttgtattct ataggtcata tcttgttttt attggcacaa atataattac
     2881 attagctttt tgagggggca ataaacagta aacacgatgg taataatggt aaaaaaaaaa
     2941 acaagcagtt atttcggata tatgtcggct actccttgcg tcgggcccga agtcttagag
     3001 ccagatatgc gagcacccgg aagctcacga tgagaatggc cagaccatga tgaaataaca
     3061 taaggtggtc ccgtcggcaa gagacatcca cttaacgtat gcttgcaata agtgcgagtg
     3121 aaaggaatag tattctgagt gtcgtattga gtctgagtga gacagcgata tgattgttga
     3181 ttaaccctta gcatgtccgt ggggtttgaa ttaactcata atattaatta gacgaaatta
     3241 tttttaaagt tttattttta ataatttgcg agtacgcaaa gcttggctgc atccaacgcg
     3301 ttgggagctc tccggatcca agcttgcatg cctgcaggtc ggagtactgt cctccgagcg
     3361 gagtactgtc ctccgagcgg agtactgtcc tccgagcgga gtactgtcct ccgagcggag
     3421 tactgtcctc cgagcggaga ctctagcgag cgccggagta taaatagagg cgcttcgtct
     3481 acggagcgac aattcaattc aaacaagcaa agtgaacacg tcgctaagcg aaagctaagc
     3541 aaataaacaa gcgcagctga acaagctaaa caatctgcag taaagtgcaa gttaaagtga
     3601 atcaattaaa agtaaccagc aaccaagtaa atcaactgca actactgaaa tctgccaaga
     3661 agtaattatt gaatacaaga agagaactct gaatagggaa ttgggaattc gttaacagat
     3721 ctgcggccgc ggctcgaggg tacctctaga ggatctttgt gaaggaacct tacttctgtg
     3781 gtgtgacata attggacaaa ctacctacag agatttaaag ctctaaggta aatataaaat
     3841 ttttaagtgt ataatgtgtt aaactactga ttctaattgt ttgtgtattt tagattccaa
     3901 cctatggaac tgatgaatgg gagcagtggt ggaatgcctt taatgaggaa aacctgtttt
     3961 gctcagaaga aatgccatct agtgatgatg aggctactgc tgactctcaa cattctactc
     4021 ctccaaaaaa gaagagaaag gtagaagacc ccaaggactt tccttcagaa ttgctaagtt
     4081 ttttgagtca tgctgtgttt agtaatagaa ctcttgcttg ctttgctatt tacaccacaa
     4141 aggaaaaagc tgcactgcta tacaagaaaa ttatggaaaa atatttgatg tatagtgcct
     4201 tgactagaga tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac
     4261 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg
     4321 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     4381 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     4441 gtctggatcg gatccactag aaggccttag tatgtatgta agttaataaa accctttttt
     4501 ggagaatgta gatttaaaaa aacatatttt ttttttattt tttactgcac tggacatcat
     4561 tgaacttatc tgatcagttt taaatttact tcgatccaag ggtatttgaa gtaccaggtt
     4621 ctttcgatta cctctcactc aaaatgacat tccactcaaa gtcagcgctg tttgcctcct
     4681 tctctgtcca cagaaatatc gccgtctctt tcgccgctgc gtccgctatc tctttcgcca
     4741 ccgtttgtag cgttacctag cgtcaatgtc cgccttcagt tgcactttgt cagcggtttc
     4801 gtgacgaagc tccaagcggt ttacgccatc aattaaacac aaagtgctgt gccaaaactc
     4861 ctctcgcttc ttatttttgt ttgttttttg agtgattggg gtggtgattg gttttgggtg
     4921 ggtaagcagg ggaaagtgtg aaaaatcccg gcaatgggcc aagaggatca ggagctatta
     4981 attcgcggag gcagcaaaca cccatctgcc gagcatctga acaatgtgag tagtacatgt
     5041 gcatacatct taagttcact tgatctatag gaactgcgat tgcaacatca aattgtctgc
     5101 ggcgtgagaa ctgcgaccca caaaaatccc aaaccgcaat cgcacaaaca aatagtgaca
     5161 cgaaacagat tattctggta gctgtgctcg ctatataaga caatttttaa gatcatatca
     5221 tgatcaagac atctaaaggc attcattttc gactacattc ttttttacaa aaaatataac
     5281 aaccagatat tttaagctga tcctagatgc acaaaaaata aataaaagta taaacctact
     5341 tcgtaggata cttcgttttg ttcggggtta gatgagcata acgcttgtag ttgatatttg
     5401 agatccccta tcattgcagg gtgacagcgg agcggcttcg cagagctgca ttaaccaggg
     5461 cttcgggcag gccaaaaact acggcacgct cctgccaccc agtccgccgg aggactccgg
     5521 ttcagggagc ggccaactag ccgagaacct cacctatgcc tggcacaata tggacatctt
     5581 tggggcggtc aatcagccgg gctccggatg gcggcagctg gtcaaccgga cacgcggact
     5641 attctgcaac gagcgacaca taccggcgcc caggaaacat ttgctcaaga acggtgagtt
     5701 tctattcgca gtcggctgat ctgtgtgaaa tcttaataaa gggtccaatt accaatttga
     5761 aactcagttt gcggcgtggc ctatccgggc gaacttttgg ccgtgatggg cagttccggt
     5821 gccggaaaga cgaccctgct gaatgccctt gcctttcgat cgccgcaggg catccaagta
     5881 tcgccatccg ggatgcgact gctcaatggc caacctgtgg acgccaagga gatgcaggcc
     5941 aggtgcgcct atgtccagca ggatgacctc tttatcggct ccctaacggc cagggaacac
     6001 ctgattttcc aggccatggt gcggatgcca cgacatctga cctatcggca gcgagtggcc
     6061 cgcgtggatc aggtgatcca ggagctttcg ctcagcaaat gtcagcacac gatcatcggt
     6121 gtgcccggca gggtgaaagg tctgtccggc ggagaaagga agcgtctggc attcgcctcc
     6181 gaggcactaa ccgatccgcc gcttctgatc tgcgatgagc ccacctccgg actggactca
     6241 tttaccgccc acagcgtcgt ccaggtgctg aagaagctgt cgcagaaggg caagaccgtc
     6301 atcctgacca ttcatcagcc gtcttccgag ctgtttgagc tctttgacaa gatccttctg
     6361 atggccgagg gcagggtagc tttcttgggc actcccagcg aagccgtcga cttcttttcc
     6421 tagtgagttc gatgtgttta ttaagggtat ctagcattac attacatctc aactcctatc
     6481 cagcgtgggt gcccagtgtc ctaccaacta caatccggcg gacttttacg tacaggtgtt
     6541 ggccgttgtg cccggacggg agatcgagtc ccgtgatcgg atcgccaaga tatgcgacaa
     6601 ttttgctatt agcaaagtag cccgggatat ggagcagttg ttggccacca aaaatttgga
     6661 gaagccactg gagcagccgg agaatgggta cacctacaag gccacctggt tcatgcagtt
     6721 ccgggcggtc ctgtggcgat cctggctgtc ggtgctcaag gaaccactcc tcgtaaaagt
     6781 gcgacttatt cagacaacgg tgagtggttc cagtggaaac aaatgatata acgcttacaa
     6841 ttcttggaaa caaattcgct agattttagt tagaattgcc tgattccaca cccttcttag
     6901 tttttttcaa tgagatgtat agtttatagt tttgcagaaa ataaataaat ttcatttaac
     6961 tcgcgaacat gttgaagata tgaatattaa tgagatgcga gtaacatttt aatttgcaga
     7021 tggttgccat cttgattggc ctcatctttt tgggccaaca actcacgcaa gtgggcgtga
     7081 tgaatatcaa cggagccatc ttcctcttcc tgaccaacat gacctttcaa aacgtctttg
     7141 ccacgataaa tgtaagtctt gtttagaata catttgcata ttaataattt actaactttc
     7201 taatgaatcg attcgattta ggtgttcacc tcagagctgc cagtttttat gagggaggcc
     7261 cgaagtcgac tttatcgctg tgacacatac tttctgggca aaacgattgc cgaattaccg
     7321 ctttttctca cagtgccact ggtcttcacg gcgattgcct atccgatgat cggactgcgg
     7381 gccggagtgc tgcacttctt caactgcctg gcgctggtca ctctggtggc caatgtgtca
     7441 acgtccttcg gatatctaat atcctgcgcc agctcctcga cctcgatggc gctgtctgtg
     7501 ggtccgccgg ttatcatacc attcctgctc tttggcggct tcttcttgaa ctcgggctcg
     7561 gtgccagtat acctcaaatg gttgtcgtac ctctcatggt tccgttacgc caacgagggt
     7621 ctgctgatta accaatgggc ggacgtggag ccgggcgaaa ttagctgcac atcgtcgaac
     7681 accacgtgcc ccagttcggg caaggtcatc ctggagacgc ttaacttctc cgccgccgat
     7741 ctgccgctgg actacgtggg tctggccatt ctcatcgtga gcttccgggt gctcgcatat
     7801 ctggctctaa gacttcgggc ccgacgcaag gagtagccga catatatccg aaataactgc
     7861 ttgttttttt ttttaccatt attaccatcg tgtttactgt ttattgcccc ctcaaaaagc
     7921 taatgtaatt atatttgtgc caataaaaac aagatatgac ctatagaata caagtatttc
     7981 cccttcgaac atccccacaa gtagactttg gatttgtctt ctaaccaaaa gacttacaca
     8041 cctgcatacc ttacatcaaa aactcgttta tcgctacata aaacaccggg atatattttt
     8101 tatatacata cttttcaaat cgcgcgccct cttcataatt cacctccacc acaccacgtt
     8161 tcgtagttgc tctttcgctg tctcccaccc gctctccgca acacattcac cttttgttcg
     8221 acgaccttgg agcgactgtc gttagttccg cgcgattcgg ttcgctcaaa tggttccgag
     8281 tggttcattt cgtctcaata gaaattagta ataaatattt gtatgtacaa tttatttgct
     8341 ccaatatatt tgtatatatt tccctcacag ctatatttat tctaatttaa tattatgact
     8401 ttttaaggta attttttgtg acctgttcgg agtgattagc gttacaattt gaactgaaag
     8461 tgacatccag tgtttgttcc ttgtgtagat gcatctcaaa aaaatggtgg gcataatagt
     8521 gttgtttata tatatcaaaa ataacaacta taataataag aatacattta atttagaaaa
     8581 tgcttggatt tcactggaac tagaattaat tcggctgctg ctctaaacga cgcatttcgt
     8641 actccaaagt acgaattttt tccctcaagc tcttattttc attaaacaat gaacaggacc
     8701 taacgcacag tcacgttatt gtttacataa atgatttttt ttactattca aacttactct
     8761 gtttgtgtac tcccactggt atagccttct tttatctttt ctggttcagg ctctatcact
     8821 ttactaggta cggcatctgc gttgagtcgc ctccttttaa atgtctgacc ttttgcaggt
     8881 gcagccttcc actgcgaatc attaaagtgg gtatcacaaa tttgggagtt ttcaccaagg
     8941 ctgcacccaa ggctctgctc ccacaatttt ctcttaatag cacacttcgg cacgtgaatt
     9001 aattttactc cagtcacagc tttgcagcaa aatttgcaat atttcatttt tttttattcc
     9061 acgtaagggt taatgttttc aaaaaaaaat tcgtccgcac acaacctttc ctctcaacaa
     9121 gcaaacgtgc actgaattta agtgtatact tcggtaagct tcggctatcg acgggaccac
     9181 cttatgttat ttcatcatg
//
