LOCUS       sequence-90722-e        9336 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  UAS vector to express gene of interest in Drosophila.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9336)
  AUTHORS   Sapar ML, Ji H, Wang B, Poe AR, Dubey K, Ren X, Ni JQ, Han C
  TITLE     Phosphatidylserine Externalization Results from and Causes Neurite 
            Degeneration in Drosophila.
  JOURNAL   Cell Rep. 2018 Aug 28;24(9):2273-2286. doi: 
            10.1016/j.celrep.2018.07.095.
  PUBMED    30157423
REFERENCE   2  (bases 1 to 9336)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2018.07"; journalName: "Cell Rep"; date: 
            "2018-08-28- 28"; volume: "24"; issue: "9"; pages: "2273-2286"
COMMENT     Sequence Label: pACUH
FEATURES             Location/Qualifiers
     source          1..9336
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(235..823)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(315..334)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(994..1785,1786..1854))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1617..1636
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1855..1959)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2027..2045
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2083..2105)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2205..2224
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     protein_bind    2434..2503
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     misc_feature    join(3046..3076,3077..3278)
                     /label=P element 3' end
                     /note="P element 3' end"
     protein_bind    3359..3453
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    3477..3571
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        3590..3828
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     3775..3796
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     intron          3965..4030
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4160..4180
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    4452..4586
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4489..4508)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4543..4562
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4613..4631)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(5091..5162,5557..5830,5905..6559,6621..6936,7157..7288,
                     7359..7973)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLLPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     misc_feature    join(8751..9305,9306..9336)
                     /label=P element 5' end
ORIGIN
        1 ggccagaccc acgtagtcca gcggcagatc ggcggcggag aagttaagcg tctccaggat
       61 gaccttgccc gaactggggc acgtggtgtt cgacgatgtg cagctaattt cgcccggctc
      121 cacgtccgcc cattggttaa tcagcagacc ctcgttggcg taacggaacc atgagaggta
      181 cgacaaccat ttgaggtata ctggcaccga gcccgagttc aagaagaagg cgtttttcca
      241 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
      301 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
      361 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
      421 gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
      481 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
      541 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
      601 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
      661 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
      721 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
      781 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
      841 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
      901 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
      961 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     1021 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     1081 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     1141 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     1201 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     1261 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     1321 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     1381 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     1441 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     1501 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     1561 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa
     1621 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     1681 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     1741 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     1801 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     1861 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     1921 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     1981 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
     2041 gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
     2101 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
     2161 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat
     2221 tgtactgaga gtgcaccata tcgacatgcc cgccgtgacc gtcgagaacc cgctgacgct
     2281 gccccgcgta tccgcacccg ccgacgccgt cgcacgtccc gtgctcaccg tgaccaccgc
     2341 gcccagcggt ttcgagggcg agggcttccc ggtgcgccgc gcgttcgccg ggatcaacta
     2401 ccgccacctc gacccgttca tcatgatgga ccagatgggt gaggtggagt acgcgcccgg
     2461 ggagcccaag ggcacgccct ggcacccgca ccgcggcttc gagaccgtga cctacatcgt
     2521 cgatatgcgg tgtgaaatac cgcaccgaat cgcgcggaac taacgacagt cgctccaagg
     2581 tcgtcgaaca aaaggtgaat gtgttgcgga gagcgggtgg gagacagcga aagagcaact
     2641 acgaaacgtg gtgtggtgga ggtgaattat gaagagggcg cgcgatttga aaagtatgta
     2701 tataaaaaat atatcccggt gttttatgta gcgataaacg agtttttgat gtaaggtatg
     2761 caggtgtgta agtcttttgg ttagaagaca aatccaaagt ctacttgtgg ggatgttcga
     2821 aggggaaata cttgtattct ataggtcata tcttgttttt attggcacaa atataattac
     2881 attagctttt tgagggggca ataaacagta aacacgatgg taataatggt aaaaaaaaaa
     2941 acaagcagtt atttcggata tatgtcggct actccttgcg tcgggcccga agtcttagag
     3001 ccagatatgc gagcacccgg aagctcacga tgagaatggc cagaccatga tgaaataaca
     3061 taaggtggtc ccgtcggcaa gagacatcca cttaacgtat gcttgcaata agtgcgagtg
     3121 aaaggaatag tattctgagt gtcgtattga gtctgagtga gacagcgata tgattgttga
     3181 ttaaccctta gcatgtccgt ggggtttgaa ttaactcata atattaatta gacgaaatta
     3241 tttttaaagt tttattttta ataatttgcg agtacgcaaa gcttggctgc atccaacgcg
     3301 ttgggagctc tccggatcca agctggccgc ggctcgatcc gcttgcatgc ctgcaggtcg
     3361 gagtactgtc ctccgagcgg agtactgtcc tccgagcgga gtactgtcct ccgagcggag
     3421 tactgtcctc cgagcggagt actgtcctcc gagcggaagc ttgcatgcct gcaggtcgga
     3481 gtactgtcct ccgagcggag tactgtcctc cgagcggagt actgtcctcc gagcggagta
     3541 ctgtcctccg agcggagtac tgtcctccga gcggagactc tagcgagcgc cggagtataa
     3601 atagaggcgc ttcgtctacg gagcgacaat tcaattcaaa caagcaaagt gaacacgtcg
     3661 ctaagcgaaa gctaagcaaa taaacaagcg cagctgaaca agctaaacaa tctgcagtaa
     3721 agtgcaagtt aaagtgaatc aattaaaagt aaccagcaac caagtaaatc aactgcaact
     3781 actgaaatct gccaagaagt aattattgaa tacaagaaga gaactctgaa tagggaattg
     3841 ggaattcgtt aacagatctg cggccgcggc tcgagggtac ctctagagga tctttgtgaa
     3901 ggaaccttac ttctgtggtg tgacataatt ggacaaacta cctacagaga tttaaagctc
     3961 taaggtaaat ataaaatttt taagtgtata atgtgttaaa ctactgattc taattgtttg
     4021 tgtattttag attccaacct atggaactga tgaatgggag cagtggtgga atgcctttaa
     4081 tgaggaaaac ctgttttgct cagaagaaat gccatctagt gatgatgagg ctactgctga
     4141 ctctcaacat tctactcctc caaaaaagaa gagaaaggta gaagacccca aggactttcc
     4201 ttcagaattg ctaagttttt tgagtcatgc tgtgtttagt aatagaactc ttgcttgctt
     4261 tgctatttac accacaaagg aaaaagctgc actgctatac aagaaaatta tggaaaaata
     4321 tttgatgtat agtgccttga ctagagatca taatcagcca taccacattt gtagaggttt
     4381 tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa
     4441 ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     4501 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     4561 tcaatgtatc ttatcatgtc tggatccact agaaggccta attctagtat gtatgtaagt
     4621 taataaaacc cttttttgga gaatgtagat ttaaaaaaac atattttttt tttatttttt
     4681 actgcactgg acatcattga acttatctga tcagttttaa atttacttcg atccaagggt
     4741 atttgaagta ccaggttctt tcgattacct ctcactcaaa atgacattcc actcaaagtc
     4801 agcgctgttt gcctccttct ctgtccacag aaatatcgcc gtctctttcg ccgctgcgtc
     4861 cgctatctct ttcgccaccg tttgtagcgt tacctagcgt caatgtccgc cttcagttgc
     4921 actttgtcag cggtttcgtg acgaagctcc aagcggttta cgccatcaat taaacacaaa
     4981 gtgctgtgcc aaaactcctc tcgcttctta tttttgtttg ttttttgagt gattggggtg
     5041 gtgattggtt ttgggtgggt aagcagggga aagtgtgaaa aatcccggca atgggccaag
     5101 aggatcagga gctattaatt cgcggaggca gcaaacaccc atctgccgag catctgaaca
     5161 atgtgagtag tacatgtgca tacatcttaa gttcacttga tctataggaa ctgcgattgc
     5221 aacatcaaat tgtctgcggc gtgagaactg cgacccacaa aaatcccaaa ccgcaatcgc
     5281 acaaacaaat agtgacacga aacagattat tctggtagct gtgctcgcta tataagacaa
     5341 tttttaagat catatcatga tcaagacatc taaaggcatt cattttcgac tacattcttt
     5401 tttacaaaaa atataacaac cagatatttt aagctgatcc tagatgcaca aaaaataaat
     5461 aaaagtataa acctacttcg taggatactt cgttttgttc ggggttagat gagcataacg
     5521 cttgtagttg atatttgaga tcccctatca ttgcagggtg acagcggagc ggcttcgcag
     5581 agctgcatta accagggctt cgggcaggcc aaaaactacg gcacgctcct gccacccagt
     5641 ccgccggagg actccggttc agggagcggc caactagccg agaacctcac ctatgcctgg
     5701 cacaatatgg acatctttgg ggcggtcaat cagccgggct ccggatggcg gcagctggtc
     5761 aaccggacac gcggactatt ctgcaacgag cgacacatac cggcgcccag gaaacatttg
     5821 ctcaagaacg gtgagtttct attcgcagtc ggctgatctg tgtgaaatct taataaaggg
     5881 tccaattacc aatttgaaac tcagtttgcg gcgtggccta tccgggcgaa cttttggccg
     5941 tgatgggcag ttccggtgcc ggaaagacga ccctgctgaa tgcccttgcc tttcgatcgc
     6001 cgcagggcat ccaagtatcg ccatccggga tgcgactgct caatggccaa cctgtggacg
     6061 ccaaggagat gcaggccagg tgcgcctatg tccagcagga tgacctcttt atcggctccc
     6121 taacggccag ggaacacctg attttccagg ccatggtgcg gatgccacga catctgacct
     6181 atcggcagcg agtggcccgc gtggatcagg tgatccagga gctttcgctc agcaaatgtc
     6241 agcacacgat catcggtgtg cccggcaggg tgaaaggtct gtccggcgga gaaaggaagc
     6301 gtctggcatt cgcctccgag gcactaaccg atccgccgct tctgatctgc gatgagccca
     6361 cctccggact ggactcattt accgcccaca gcgtcgtcca ggtgctgaag aagctgtcgc
     6421 agaagggcaa gaccgtcatc ctgaccattc atcagccgtc ttccgagctg tttgagctct
     6481 ttgacaagat ccttctgatg gccgagggca gggtagcttt cttgggcact cccagcgaag
     6541 ccgtcgactt cttttcctag tgagttcgat gtgtttatta agggtatcta gcattacatt
     6601 acatctcaac tcctatccag cgtgggtgcc cagtgtccta ccaactacaa tccggcggac
     6661 ttttacgtac aggtgttggc cgttgtgccc ggacgggaga tcgagtcccg tgatcggatc
     6721 gccaagatat gcgacaattt tgctattagc aaagtagccc gggatatgga gcagttgttg
     6781 gccaccaaaa atttggagaa gccactggag cagccggaga atgggtacac ctacaaggcc
     6841 acctggttca tgcagttccg ggcggtcctg tggcgatcct ggctgtcggt gctcaaggaa
     6901 ccactcctcg taaaagtgcg acttattcag acaacggtga gtggttccag tggaaacaaa
     6961 tgatataacg cttacaattc ttggaaacaa attcgctaga ttttagttag aattgcctga
     7021 ttccacaccc ttcttagttt ttttcaatga gatgtatagt ttatagtttt gcagaaaata
     7081 aataaatttc atttaactcg cgaacatgtt gaagatatga atattaatga gatgcgagta
     7141 acattttaat ttgcagatgg ttgccatctt gattggcctc atctttttgg gccaacaact
     7201 cacgcaagtg ggcgtgatga atatcaacgg agccatcttc ctcttcctga ccaacatgac
     7261 ctttcaaaac gtctttgcca cgataaatgt aagtcttgtt tagaatacat ttgcatatta
     7321 ataatttact aactttctaa tgaatcgatt cgatttaggt gttcacctca gagctgccag
     7381 tttttatgag ggaggcccga agtcgacttt atcgctgtga cacatacttt ctgggcaaaa
     7441 cgattgccga attaccgctt tttctcacag tgccactggt cttcacggcg attgcctatc
     7501 cgatgatcgg actgcgggcc ggagtgctgc acttcttcaa ctgcctggcg ctggtcactc
     7561 tggtggccaa tgtgtcaacg tccttcggat atctaatatc ctgcgccagc tcctcgacct
     7621 cgatggcgct gtctgtgggt ccgccggtta tcataccatt cctgctcttt ggcggcttct
     7681 tcttgaactc gggctcggtg ccagtatacc tcaaatggtt gtcgtacctc tcatggttcc
     7741 gttacgccaa cgagggtctg ctgattaacc aatgggcgga cgtggagccg ggcgaaatta
     7801 gctgcacatc gtcgaacacc acgtgcccca gttcgggcaa ggtcatcctg gagacgctta
     7861 acttctccgc cgccgatctg ccgctggact acgtgggtct ggccattctc atcgtgagct
     7921 tccgggtgct cgcatatctg gctctaagac ttcgggcccg acgcaaggag tagccgacat
     7981 atatccgaaa taactgcttg tttttttttt taccattatt accatcgtgt ttactgttta
     8041 ttgccccctc aaaaagctaa tgtaattata tttgtgccaa taaaaacaag atatgaccta
     8101 tagaatacaa gtatttcccc ttcgaacatc cccacaagta gactttggat ttgtcttcta
     8161 accaaaagac ttacacacct gcatacctta catcaaaaac tcgtttatcg ctacataaaa
     8221 caccgggata tattttttat atacatactt ttcaaatcgc gcgccctctt cataattcac
     8281 ctccaccaca ccacgtttcg tagttgctct ttcgctgtct cccacccgct ctccgcaaca
     8341 cattcacctt ttgttcgacg accttggagc gactgtcgtt agttccgcgc gattcggttc
     8401 gctcaaatgg ttccgagtgg ttcatttcgt ctcaatagaa attagtaata aatatttgta
     8461 tgtacaattt atttgctcca atatatttgt atatatttcc ctcacagcta tatttattct
     8521 aatttaatat tatgactttt taaggtaatt ttttgtgacc tgttcggagt gattagcgtt
     8581 acaatttgaa ctgaaagtga catccagtgt ttgttccttg tgtagatgca tctcaaaaaa
     8641 atggtgggca taatagtgtt gtttatatat atcaaaaata acaactataa taataagaat
     8701 acatttaatt tagaaaatgc ttggatttca ctggaactag aattaattcg gctgctgctc
     8761 taaacgacgc atttcgtact ccaaagtacg aattttttcc ctcaagctct tattttcatt
     8821 aaacaatgaa caggacctaa cgcacagtca cgttattgtt tacataaatg atttttttta
     8881 ctattcaaac ttactctgtt tgtgtactcc cactggtata gccttctttt atcttttctg
     8941 gttcaggctc tatcacttta ctaggtacgg catctgcgtt gagtcgcctc cttttaaatg
     9001 tctgaccttt tgcaggtgca gccttccact gcgaatcatt aaagtgggta tcacaaattt
     9061 gggagttttc accaaggctg cacccaaggc tctgctccca caattttctc ttaatagcac
     9121 acttcggcac gtgaattaat tttactccag tcacagcttt gcagcaaaat ttgcaatatt
     9181 tcattttttt ttattccacg taagggttaa tgttttcaaa aaaaaattcg tccgcacaca
     9241 acctttcctc tcaacaagca aacgtgcact gaatttaagt gtatacttcg gtaagcttcg
     9301 gctatcgacg ggaccacctt atgttatttc atcatg
//
