LOCUS       sequence-90723-e         654 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin, Excitation: 559, Emission: 600.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 654)
  AUTHORS   Lam AJ, St-Pierre F, Gong Y, Marshall JD, Cranfill PJ, Baird MA, 
            McKeown MR, Wiedenmann J, Davidson MW, Schnitzer MJ, Tsien RY, Lin 
            MZ
  TITLE     Improving FRET dynamic range with bright green and red fluorescent 
            proteins.
  JOURNAL   Nat Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171.
  PUBMED    22961245
REFERENCE   2  (bases 1 to 654)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171."
COMMENT     Sequence Label: mRuby2-Lifeact-7
FEATURES             Location/Qualifiers
     source          1..654
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      24..33
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             30..80
                     /codon_start=1
                     /product="peptide that binds filamentous actin (Riedl et 
                     al., 2008)"
                     /label=Lifeact
                     /note="first 17 amino acids of budding yeast Abp140"
                     /translation="MGVADLIKKFESISKEE"
     regulatory      96..105
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 agtgaaccgt cagatccgct agcgccacca tgggcgtggc cgacttgatc aagaagttcg
       61 agtccatctc caaggaggag ggggatccac cggtcgccac catggtgtct aagggcgaag
      121 agctgatcaa ggaaaatatg cgtatgaagg tggtcatgga aggttcggtc aacggccacc
      181 aattcaaatg cacaggtgaa ggagaaggca atccgtacat gggaactcaa accatgagga
      241 tcaaagtcat cgagggagga cccctgccat ttgcctttga cattcttgcc acgtcgttca
      301 tgtatggcag ccgtactttt atcaagtacc cgaaaggcat tcctgatttc tttaaacagt
      361 cctttcctga gggttttact tgggaaagag ttacgagata cgaagatggt ggagtcgtca
      421 ccgtcatgca ggacaccagc cttgaggatg gctgtctcgt ttaccacgtc caagtcagag
      481 gggtaaactt tccctccaat ggtcccgtga tgcagaagaa gaccaagggt tgggagccta
      541 atacagagat gatgtatcca gcagatggtg gtctgagggg atacactcat atggcactga
      601 aagttgatgg tggtggccat ctgtcttgct ctttcgtaac aacttacagg tcaa
//
