LOCUS       sequence-90724-e       10481 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  UAS vector to express gene of interest in Drosophila.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10481)
  AUTHORS   Sapar ML, Ji H, Wang B, Poe AR, Dubey K, Ren X, Ni JQ, Han C
  TITLE     Phosphatidylserine Externalization Results from and Causes Neurite 
            Degeneration in Drosophila.
  JOURNAL   Cell Rep. 2018 Aug 28;24(9):2273-2286. doi: 
            10.1016/j.celrep.2018.07.095.
  PUBMED    30157423
REFERENCE   2  (bases 1 to 10481)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2018.07"; journalName: "Cell Rep"; date: 
            "2018-08-28- 28"; volume: "24"; issue: "9"; pages: "2273-2286"
COMMENT     Sequence Label: pIHEU-MCS
FEATURES             Location/Qualifiers
     source          1..10481
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    join(1..31,32..233)
                     /label=P element 3' end
                     /note="P element 3' end"
     protein_bind    631..1016
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     protein_bind    1044..1138
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        1157..1395
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     1342..1363
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     CDS             1406..1417
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     intron          1468..1600
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1583..1602
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     protein_bind    2337..2722
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     primer_bind     complement(2732..2750)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     misc_feature    join(6851..7405,7406..7436)
                     /label=P element 5' end
     rep_origin      complement(7671..8259)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7751..7770)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8430..9221,9222..9290))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9053..9072
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9291..9395)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9463..9481
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(9519..9541)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     9641..9660
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     protein_bind    9869..9938
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
ORIGIN
        1 catgatgaaa taacataagg tggtcccgtc ggcaagagac atccacttaa cgtatgcttg
       61 caataagtgc gagtgaaagg aatagtattc tgagtgtcgt attgagtctg agtgagacag
      121 cgatatgatt gttgattaac ccttagcatg tccgtggggt ttgaattaac tcataatatt
      181 aattagacga aattattttt aaagttttat ttttaataat ttgcgagtac gcaaagcttc
      241 ctaggtcaag accgacctgt ccggtgccct gaatgaactg caagacgagg cagcgcggct
      301 atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc
      361 gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct
      421 tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga
      481 tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg
      541 gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc
      601 agccgaactg ttcgccaagc ttggctgcat cacgtaataa gtgtgcgttg aatttattcg
      661 caaaaacatt gcatattttc ggcaaagtaa aattttgttg cataccttat caaaaaataa
      721 gtgctgcata ctttttagag aaaccaaata attttttatt gcatacccgt ttttaataaa
      781 atacattgca taccctcttt taataaaaaa tattgcatac tttgacgaaa caaattttcg
      841 ttgcataccc aataaaagat tattatattg catacccgtt tttaataaaa tacattgcat
      901 accctctttt aataaaaaat attgcatacg ttgacgaaac aaattttcgt tgcataccca
      961 ataaaagatt attatattgc ataccttttc ttgccatacc atttagccga tcaattgtgc
     1021 tcggcaacag catgcctgca ggtcggagta ctgtcctccg agcggagtac tgtcctccga
     1081 gcggagtact gtcctccgag cggagtactg tcctccgagc ggagtactgt cctccgagcg
     1141 gagactctag cgagcgccgg agtataaata gaggcgcttc gtctacggag cgacaattca
     1201 attcaaacaa gcaaagtgaa cacgtcgcta agcgaaagct aagcaaataa acaagcgcag
     1261 ctgaacaagc taaacaatct gcagtaaagt gcaagttaaa gtgaatcaat taaaagtaac
     1321 cagcaaccaa gtaaatcaac tgcaactact gaaatctgcc aagaagtaat tattgaatac
     1381 aagaagagaa ctctgaatag ggaattggga attgcagtga ctctcttaag gtagccttgc
     1441 agaagttggt cgtgaggcac tgggcaggta agtatcaagg ttacaagaca ggtttaagga
     1501 gaccaataga aactgggctt gtcgagacag agaagactct tgcgtttctg ataggcacct
     1561 attggtctta ctgacatcca ctttgccttt ctctccacag gtgtccactc ccagttcaat
     1621 tacagctcga attcgttaac agatctgcgg ccgcggctcg agggtacctc tagaattaat
     1681 taactaagcc agtcggcaat cggacgcctt cgaaacatgc aacactaatg tttaattcag
     1741 atttcagcag agacaagcta aaacaccgac gagttgtaat catttctgtg cgccagcata
     1801 tatttcttat atacaacgta atacataatt atgtaattct agcatctccc caacactcac
     1861 atacaaacaa acaaaaaata caaacacaca aaacgtattt acccgcacgc atccttggcg
     1921 aggttgagta tgaaacaaaa acaaaactta atttagagca aagtaattac acgaataaat
     1981 ttaataaaaa aaaactataa taaaaacgcc aatcatgtta tttccaaaaa aaaaaaaaaa
     2041 aaaaaaaact agacgagatg tttgcgcttg tgtcgatgaa tgtaaagaat tgcaactaca
     2101 tagatggtaa ttaatttcag ctgtaagaag tacaagtttt tcataattgt gagaaaagtg
     2161 cagtagtatc gcttaaagtt cagttttaat cagggcattt agcagctcga acccaatgta
     2221 tttgtaagcc caataaacgt gtaaatgcct agagcaaaat atcacaactg tagcttttca
     2281 tccgaaagat aataatgaat agactgacct gacgtttttc aaaatgcgta actagtcacg
     2341 taataagtgt gcgttgaatt tattcgcaaa aacattgcat attttcggca aagtaaaatt
     2401 ttgttgcata ccttatcaaa aaataagtgc tgcatacttt ttagagaaac caaataattt
     2461 tttattgcat acccgttttt aataaaatac attgcatacc ctcttttaat aaaaaatatt
     2521 gcatactttg acgaaacaaa ttttcgttgc atacccaata aaagattatt atattgcata
     2581 cccgttttta ataaaataca ttgcataccc tcttttaata aaaaatattg catacgttga
     2641 cgaaacaaat tttcgttgca tacccaataa aagattatta tattgcatac cttttcttgc
     2701 cataccattt agccgatcaa ttctagtatg tatgtaagtt aataaaaccc atttttgcgg
     2761 aaagtagata aaaaaaacat tttttttttt tactgcactg gatatcattg aacttatctg
     2821 atcagtttta aatttacttc gatccaaggg tatttgatgt accaggttct ttcgattacc
     2881 tctcactcaa aatgacattc cactcaaagt cagcgctgtt tgcctccttc tctgtccaca
     2941 gaaatatcgc cgtctctttc gccgctgcgt ccgctatctc tttcgccacc gtttgtagcg
     3001 ttacgtagcg tcaatgtccg ccttcagttg cattttgtca gcggtttcgt gacgaagctc
     3061 caagcggttt acgccatcaa ttaaacacaa agtgctgtgc caaaactcct ctcgcttctt
     3121 atttttgttt gttttttgag tgattggggt ggtgattggt tttgggtggg taagcagggg
     3181 aaagtgtgaa aaatcccggc aatgggccaa gaggatcagg agctattaat tcgcggaggc
     3241 agcaaacacc catctgccga gcatctgaac aatgtgagta gtacatgtgc atacatctta
     3301 agttcacttg atctatagga actgcgattg caacatcaaa ttgtctgcgg cgtgagaact
     3361 gcgacccaca aaaatcccaa accgcaatcg cacaaacaaa tagtgacacg aaacagatta
     3421 ttctggtagc tgttctcgct atataagaca atttttgaga tcatatcatg atcaagacat
     3481 ctaaaggcat tcattttcga ctatattctt ttttacaaaa aatataacaa ccagatattt
     3541 taagctgatc ctagatgcac aaaaaataaa taaaagtata aacctacttc gtaggatact
     3601 tcggggtact ttttgttcgg ggttagatga gcataacgct tgtagttgat atttgagatc
     3661 ccctatcatt gcagggtgac agcggacgct tcgcagagct gcattaacca gggcttcggg
     3721 caggccaaaa actacggcac gctccggcca cccagtccgc cggaggactc cggttcaggg
     3781 agcggccaac tagccgagaa cctcacctat gcctggcaca atatggacat ctttggggcg
     3841 gtcaatcagc cgggtcccgg atggcggcag ctggtcaacc ggacacgcgg actattctgc
     3901 aacgagcgac acataccggc gcccaggaaa catttgctca agaacggtga gtttctattc
     3961 gcagtcggct gatctgtgtg aaatcttaat aaagggtcca attaccaatt tgaaactcag
     4021 tttgcggcgt ggcctatccg ggcgaacttt tggccgtgat gggcagttcc ggtgccggaa
     4081 agacgaccct gctgaatgcc cttgcctttc gatcgccgca gggcatccaa gtatcgccat
     4141 ccgggatgcg actgctcaat ggccaacctg tggacgccaa ggagatgcag gccaggtgcg
     4201 cctatgtcca gcaggatgac ctctttatcg gctccctaac ggccagggaa cacctgattt
     4261 tccaagccat ggtgcggatg ccacgacatc tgacctatcg gcagcgagtg gcccgcgtgg
     4321 atcaggtgat ccaggagctt tcgctcagca aatgtcagca cacgatcatc ggtgtgcccg
     4381 gcagggtgaa aggtctgtcc ggcggagaaa ggaagcgtct ggcattcgcc tccgaggctc
     4441 taaccgatcc gccgcttctg atctgcgatg agcccacctc cggactggac tcctttaccg
     4501 cccacagcgt cgtccaggtg ctgaagaagc tgtcgcagaa gggcaagacc gtcatcctga
     4561 ccattcatca gccgtcttcc gagctgtttg agctctttga caagatcctt ctgatggccg
     4621 agggcagggt agctttcttg ggcactccca gcgaagccgt cgacttcttt tcctagtgag
     4681 ttcgatgtgt ttattaaggg tatctagtat tacataacat ctcaactcct atccagcgtg
     4741 ggtgcccagt gtcctaccaa ctacaatccg gcggactttt acgtacaggt gttggccgtt
     4801 gtgcccggac gggagatcga gtcccgtgat cggatcgcca agatatgcga caattttgcc
     4861 attagcaaag tagcccggga tatggagcag ttgttggcca ccaaaaatct ggagaagcca
     4921 ctggagcagc cggagaatgg gtacacctac aaggccacct ggttcatgca gttccgggcg
     4981 gtcctgtggc gatcctggct gtcggtgctc aaggaaccac tcctcgtaaa agtgcgactt
     5041 attcagacaa cggtgagtgg ttccagtgga aacaaatgat ataacgctta caattcttgg
     5101 aaacaaattc gctagatttt agatagaatt gcctgattcc acacccttct tagttttttt
     5161 caatgagatg tatagtttat agttttgcag aagataaata aatttcattt aactcgcgaa
     5221 tattaatgag atgcgagtaa cattttaatt tgcagatggt tgccatcttg attggcctca
     5281 tctttttggg ccaacaactc acgcaagtgg gtgtgatgaa tatcaacgga gccatcttcc
     5341 tcttcctgac caacatgacc tttcaaaacg tctttgccac gataaatgta agtcatgttt
     5401 agaatacatt tgcatttcaa taatttacta actttctaat gaatcgattc gatttaggtg
     5461 ttcacctcag agctgccagt ttttatgagg gaggcccgaa gtcgacttta tcgctgtgac
     5521 acatactttc tgggcaaaac gattgccgaa ttgccgcttt ttctcacagt gccactggtc
     5581 ttcacggcga ttgcctatcc gatgatcgga ctgcgggccg gagtgctgca cttcttcaac
     5641 tgcctggcgc tggtcactct ggtggccaat gtgtcaacgt ccttcggata tctaatatcc
     5701 tgcgccagct cctcgacctc gatggcgctg tctgtgggtc cgccggttat cataccattc
     5761 ctgctctttg gcggcttctt cttgaactcg ggctcggtgc cagtatacct caaatggttg
     5821 tcgtacctct catggttccg ttacgccaac gagggtctgc tgattaacca atgggcggac
     5881 gtggagccgg gcgaaattag ctgcacatcg tcgaacacca cgtgccccag ttcgggcaag
     5941 gtcatcctgg agacgcttaa cttctccgcc gccgatctgc cgctggacta cgtgggtctg
     6001 gccattctca tcgtgagctt ccgggtgctc gcatatctgg ctctaagact tcgggcccga
     6061 cgcaaggagt agccgacata tatccgaaat aactgcttgt tttttttttt taccattatt
     6121 accatcgtgt ttactgttta ttgccccctc aaaaagctaa tgtaattata tttgtgccaa
     6181 taaaaacaag atatgaccta tagaatacaa gtatttcccc ttcgaacatc cccacaagta
     6241 gactttggat ttgtcttcta accaaaagac ttacacacct gcatacctta catcaaaaac
     6301 tcgtttatcg ctacataaaa caccgggata tattttttat atacatactt ttcaaatcgc
     6361 gcgccctctt cataattcac ctccaccaca ccacgtttcg tagttgctct ttcgctgtct
     6421 cccacccgct ctccgcaaca cattcacctt ttgttcgacg accttggagc gactgtcgtt
     6481 agttccgcgc gattcggttc gctcaaatgg ttccgagtgg ttcatttcgt ctcaatagaa
     6541 attagtaata aatatttgta tgtacaattt atttgctcca atatatttgt atatatttcc
     6601 ctcacagcta tatttattct aatttaatat tatgactttt taaggtaatt ttttgtgacc
     6661 tgttcggagt gattagcgtt acaatttgaa ctgaaagtga catccagtgt ttgttccttg
     6721 tgtagatgca tctcaaaaaa atggtgggca taatagtgtt gtttatatat atcaaaaata
     6781 acaactataa taataagaat acatttaatt tagaaaatgc ttggatttca ctggaactag
     6841 aattaattcg gctgctgctc taaacgacgc atttcgtact ccaaagtacg aattttttcc
     6901 ctcaagctct tattttcatt aaacaatgaa caggacctaa cgcacagtca cgttattgtt
     6961 tacataaatg atttttttta ctattcaaac ttactctgtt tgtgtactcc cactggtata
     7021 gccttctttt atcttttctg gttcaggctc tatcacttta ctaggtacgg catctgcgtt
     7081 gagtcgcctc cttttaaatg tctgaccttt tgcaggtgca gccttccact gcgaatcatt
     7141 aaagtgggta tcacaaattt gggagttttc accaaggctg cacccaaggc tctgctccca
     7201 caattttctc ttaatagcac acttcggcac gtgaattaat tttactccag tcacagcttt
     7261 gcagcaaaat ttgcaatatt tcattttttt ttattccacg taagggttaa tgttttcaaa
     7321 aaaaaattcg tccgcacaca acctttcctc tcaacaagca aacgtgcact gaatttaagt
     7381 gtatacttcg gtaagcttcg gctatcgacg ggaccacctt atgttatttc atcatgggcc
     7441 agacccacgt agtccagcgg cagatcggcg gcggagaagt taagcgtctc caggatgacc
     7501 ttgcccgaac tggggcacgt ggtgttcgac gatgtgcagc taatttcgcc cggctccacg
     7561 tccgcccatt ggttaatcag cagaccctcg ttggcgtaac ggaaccatga gaggtacgac
     7621 aaccatttga ggtatactgg caccgagccc gagttcaaga agaaggcgtt tttccatagg
     7681 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     7741 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     7801 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     7861 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     7921 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     7981 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     8041 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     8101 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     8161 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     8221 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     8281 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     8341 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     8401 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     8461 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     8521 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     8581 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     8641 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     8701 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     8761 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     8821 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     8881 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     8941 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     9001 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaacacg ggataatacc
     9061 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     9121 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     9181 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     9241 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     9301 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     9361 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     9421 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg
     9481 ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg
     9541 gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg
     9601 tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta
     9661 ctgagagtgc accattcgac atgcccgccg tgaccgtcga gaacccgctg acgctgcccc
     9721 gcgtatccgc acccgccgac gccgtcgcac gtcccgtgct caccgtgacc accgcgccca
     9781 gcggtttcga gggcgagggc ttcccggtgc gccgcgcgtt cgccgggatc aactaccgcc
     9841 acctcgaccc gttcatcatg atggaccaga tgggtgaggt ggagtacgcg cccggggagc
     9901 ccaagggcac gccctggcac ccgcaccgcg gcttcgagac cgtgacctac atcgtcgata
     9961 tgcggtgtga aataccgcac cgaatcgcgc ggaactaacg acagtcgctc caaggtcgtc
    10021 gaacaaaagg tgaatgtgtt gcggagagcg ggtgggagac agcgaaagag caactacgaa
    10081 acgtggtgtg gtggaggtga attatgaaga gggcgcgcga tttgaaaagt atgtatataa
    10141 aaaatatatc ccggtgtttt atgtagcgat aaacgagttt ttgatgtaag gtatgcaggt
    10201 gtgtaagtct tttggttaga agacaaatcc aaagtctact tgtggggatg ttcgaagggg
    10261 aaatacttgt attctatagg tcatatcttg tttttattgg cacaaatata attacattag
    10321 ctttttgagg gggcaataaa cagtaaacac gatggtaata atggtaaaaa aaaaaaacaa
    10381 gcagttattt cggatatatg tcggctactc cttgcgtcgg gcccgaagtc ttagagccag
    10441 atatgcgagc acccggaagc tcacgatgag aatggccaga c
//
