LOCUS       sequence-90729-e        2373 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses truncated C-terminal SpCas9 domain fused to a GyrA intein,
            flanked by ITRs for AAV packaging. Combine with N-Terminal Split 
            Cas9 Gyra Intein for full length SpCas9 production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2373)
  AUTHORS   Fine EJ, Appleton CM, White DE, Brown MT, Deshmukh H, Kemp ML, Bao G
  TITLE     Trans-spliced Cas9 allows cleavage of HBB and CCR5 genes in human 
            cells using compact expression cassettes.
  JOURNAL   Sci Rep. 2015 Jul 1;5:10777. doi: 10.1038/srep10777.
  PUBMED    26126518
REFERENCE   2  (bases 1 to 2373)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2015-07-1"; volume: "5:10777. doi"; pages: " 
            10.1038/srep10777"
COMMENT     Sequence Label: C-Terminal Split Cas9 with GyrA intein
FEATURES             Location/Qualifiers
     source          1..2373
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ggaaaaccgg aatttgcccc aactacatat actgtcggcg tcccagggct ggttcgattc
       61 ctggaagccc accacaggga tccagacgct caggcaatcg cagacgagct gaccgacggg
      121 aggttttatt atgctaaggt cgcctctgtg accgatgcag gcgtacagcc tgtgtattct
      181 cttagggtgg acacagccga ccatgctttt attaccaatg gcttcgttag tcacaatacc
      241 ggctggggca ggctgagccg gaagctgatc aacggcatcc gggacaagca gtccggcaag
      301 acaatcctgg atttcctgaa gtccgacggc ttcgccaaca gaaacttcat gcagctgatc
      361 cacgacgaca gcctgacctt taaagaggac atccagaaag cccaggtgtc cggccagggc
      421 gatagcctgc acgagcacat tgccaatctg gccggcagcc ccgccattaa gaagggcatc
      481 ctgcagacag tgaaggtggt ggacgagctc gtgaaagtga tgggccggca caagcccgag
      541 aacatcgtga tcgaaatggc cagagagaac cagaccaccc agaagggaca gaagaacagc
      601 cgcgagagaa tgaagcggat cgaagagggc atcaaagagc tgggcagcca gatcctgaaa
      661 gaacaccccg tggaaaacac ccagctgcag aacgagaagc tgtacctgta ctacctgcag
      721 aatgggcggg atatgtacgt ggaccaggaa ctggacatca accggctgtc cgactacgat
      781 gtggaccata tcgtgcctca gagctttctg aaggacgact ccatcgacaa caaggtgctg
      841 accagaagcg acaagaaccg gggcaagagc gacaacgtgc cctccgaaga ggtcgtgaag
      901 aagatgaaga actactggcg gcagctgctg aacgccaagc tgattaccca gagaaagttc
      961 gacaatctga ccaaggccga gagaggcggc ctgagcgaac tggataaggc cggcttcatc
     1021 aagagacagc tggtggaaac ccggcagatc acaaagcacg tggcacagat cctggactcc
     1081 cggatgaaca ctaagtacga cgagaatgac aagctgatcc gggaagtgaa agtgatcacc
     1141 ctgaagtcca agctggtgtc cgatttccgg aaggatttcc agttttacaa agtgcgcgag
     1201 atcaacaact accaccacgc ccacgacgcc tacctgaacg ccgtcgtggg aaccgccctg
     1261 atcaaaaagt accctaagct ggaaagcgag ttcgtgtacg gcgactacaa ggtgtacgac
     1321 gtgcggaaga tgatcgccaa gagcgagcag gaaatcggca aggctaccgc caagtacttc
     1381 ttctacagca acatcatgaa ctttttcaag accgagatta ccctggccaa cggcgagatc
     1441 cggaagcggc ctctgatcga gacaaacggc gaaaccgggg agatcgtgtg ggataagggc
     1501 cgggattttg ccaccgtgcg gaaagtgctg agcatgcccc aagtgaatat cgtgaaaaag
     1561 accgaggtgc agacaggcgg cttcagcaaa gagtctatcc tgcccaagag gaacagcgat
     1621 aagctgatcg ccagaaagaa ggactgggac cctaagaagt acggcggctt cgacagcccc
     1681 accgtggcct attctgtgct ggtggtggcc aaagtggaaa agggcaagtc caagaaactg
     1741 aagagtgtga aagagctgct ggggatcacc atcatggaaa gaagcagctt cgagaagaat
     1801 cccatcgact ttctggaagc caagggctac aaagaagtga aaaaggacct gatcatcaag
     1861 ctgcctaagt actccctgtt cgagctggaa aacggccgga agagaatgct ggcctctgcc
     1921 ggcgaactgc agaagggaaa cgaactggcc ctgccctcca aatatgtgaa cttcctgtac
     1981 ctggccagcc actatgagaa gctgaagggc tcccccgagg ataatgagca gaaacagctg
     2041 tttgtggaac agcacaagca ctacctggac gagatcatcg agcagatcag cgagttctcc
     2101 aagagagtga tcctggccga cgctaatctg gacaaagtgc tgtccgccta caacaagcac
     2161 cgggataagc ccatcagaga gcaggccgag aatatcatcc acctgtttac cctgaccaat
     2221 ctgggagccc ctgccgcctt caagtacttt gacaccacca tcgaccggaa gaggtacacc
     2281 agcaccaaag aggtgctgga cgccaccctg atccaccaga gcatcaccgg cctgtacgag
     2341 acacggatcg acctgtctca gctgggaggc gac
//
