LOCUS       sequence-90737-e         602 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  allows Cre-mediated expression of transgene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 602)
  AUTHORS   Nakamura T, Gulick J, Pratt R, Robbins J
  TITLE     Noonan syndrome is associated with enhanced pERK activity, the 
            repression of which can prevent craniofacial malformations.
  JOURNAL   Proc Natl Acad Sci U S A. 2009 Sep 8;106(36):15436-41. doi: 
            10.1073/pnas.0903302106. Epub 2009 Aug 24.
  PUBMED    19706403
REFERENCE   2  (bases 1 to 602)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.0903302106"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2009-09-8- 8"; volume: "106"; issue: "36"; pages: "15436-41"
COMMENT     Sequence Label: CAG-lox-CAT-lox-unique Bam cloning site-hGH po...
FEATURES             Location/Qualifiers
     source          1..602
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    93..126
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(177..196)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     complement(258..280)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
ORIGIN
        1 ggtatatcca gtgatttttt tctccatttt agcttcctta gctcctgaaa atctcgccaa
       61 gctcctcgag atccagatcc ggaaccctta atataacttc gtataatgta tgctatacga
      121 agttattagg tccctcgacc gcccagcttg aattctttgc caaaatgatg agacagcaca
      181 ataaccagca cgttgcccag gagctgtagg aaaaagaaga aggcatgaac atggttagca
      241 gaggctctag agccgccggt cacacgccag aagccgaacc ccgccctgcc ccgtcccccc
      301 cgaaggcagc cgtccccccg cggacagccc cgaggctgga gagggagaag gggacggcgg
      361 cgcggcgacg cacgaaggcc ctccccgccc atttccttcc tgccggcgcc gcaccgcttc
      421 gccccgcgcc cgctagaggg ggtgcggcgg cgcctcccag atttcggctc cgcacagatt
      481 tgggacaaag gaagtccctg cgccctctcg cacgattacc ataaaaggca atggctgcgg
      541 ctcgccgcgc ctcgacagcc gccggcgctc cgggggccgc cgcgcccctc ccccgagccc
      601 tc
//
