LOCUS       sequence-90744-e         818 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Luciferase reporter containing 13 copies of the p53-binding 
            consensus sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 818)
  AUTHORS   el-Deiry WS, Tokino T, Velculescu VE, Levy DB, Parsons R, Trent JM, 
            Lin D, Mercer WE, Kinzler KW, Vogelstein B
  TITLE     WAF1, a potential mediator of p53 tumor suppression.
  JOURNAL   Cell. 1993 Nov 19. 75(4):817-25.
  PUBMED    8242752
REFERENCE   2  (bases 1 to 818)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 1993 
            Nov 19. 75(4):817-25."
COMMENT     Sequence Label: PG13-luc (wt p53 binding sites)
FEATURES             Location/Qualifiers
     source          1..818
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        15..33
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     70..86
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     70..86
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     polyA_signal    83..217
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(107..126)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     161..180
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(642..662)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 attacgaagc tcgaaattaa ccctcactaa agggaacaaa agctggagct ccaccgcggt
       61 ggcggccgct ctagaactag tggatccaga catgataaga tacattgatg agtttggaca
      121 aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc
      181 tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt
      241 tatgtttcag gttcaggggg aggtgtggga ggttttttaa agcaagtaaa acctctacaa
      301 atgtggtatg gctgattatg atctctagtc aaggcactat acatcaaata ttccttatta
      361 acccctttac aaattaaaaa gctaaaggta cacaattttt gagcatagtt attaatagca
      421 gacactctat gcctgtgtgg agtaagaaaa aacagtatgt tatgattata actgttatgc
      481 ctacttataa aggttacaga atatttttcc ataattttct tgtatagcag tgcagctttt
      541 tcctttgtgg tgtaaatagc aaagcaagca agagttctat tactaaacac agcatgactc
      601 aaaaaactta gcaattctga aggaaagtcc ttggggtctt ctacctttct cttctttttt
      661 ggaggagtag aatgttgaga gtcagcagta gcctcatcat cactagatgg catttcttct
      721 gagcaaaaca ggttttcctc attaaaggca ttccaccact gctcccattc atcagttcca
      781 taggttggaa tctaaaatac acaaacaatt agaatcag
//
