LOCUS       sequence-90745-e         821 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Luciferase reporter containing 13 copies of the p53-binding 
            consensus sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 821)
  AUTHORS   el-Deiry WS, Tokino T, Velculescu VE, Levy DB, Parsons R, Trent JM, 
            Lin D, Mercer WE, Kinzler KW, Vogelstein B
  TITLE     WAF1, a potential mediator of p53 tumor suppression.
  JOURNAL   Cell. 1993 Nov 19. 75(4):817-25.
  PUBMED    8242752
REFERENCE   2  (bases 1 to 821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 1993 
            Nov 19. 75(4):817-25."
COMMENT     Sequence Label: PG13-luc (wt p53 binding sites)
FEATURES             Location/Qualifiers
     source          1..821
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          221..286
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             416..436
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gtaccgaaag gtcttaccgg aaaactcgac gcaagaaaaa tcagagagat cctcataaag
       61 gccaagaagg gcggaaagtc caaattgtaa aatgtaactg tattcagcga tgacgaaatt
      121 cttagctatt gtaatactct agaggatctt tgtgaaggaa ccttacttct gtggtgtgac
      181 ataattggac aaactaccta cagagattta aagctctaag gtaaatataa aatttttaag
      241 tgtataatgt gttaaactac tgattctaat tgtttgtgta ttttagattc caacctatgg
      301 aactgatgaa tgggagcagt ggtggaatgc ctttaatgag gaaaacctgt tttgctcaga
      361 agaaatgcca tctagtgatg atgaggctac tgctgactct caacattcta ctcctccaaa
      421 aaagaagaga aaggtagaag accccaagga ctttccttca gaattgctaa gttttttgag
      481 tcatgctgtg tttagtaata gaactcttgc ttgctttgct atttacacca caaaggaaaa
      541 agctgcactg ctatacaaga aaattatgga aaaatattct gtaaccttta taagtaggca
      601 taacagttat aatcataaca tactgttttt tcttactcca cacaggcata gagtgtctgc
      661 tattaataac tatgctcaaa aattgtgtac ctttagcttt ttaatttgta aaggggttaa
      721 taaggaatat ttgatgtata gtgccttgac tagagatcat aatcagccat accacatttg
      781 tagaggtttt acttgcttta aaaaacctcc cacacctccc c
//
