LOCUS       sequence-90746-e         849 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Characteristic fingerprint molecule to perform single molecule force
            spectroscopy experiments; covalently immobilized via ybbr tag .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Otten M, Ott W, Jobst MA, Milles LF, Verdorfer T, Pippig DA, Nash 
            MA, Gaub HE
  TITLE     From genes to protein mechanics on a chip.
  JOURNAL   Nat Methods. 2014 Sep 7. doi: 10.1038/nmeth.3099.
  PUBMED    25194847
REFERENCE   2  (bases 1 to 849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2014 Sep 7. doi: 10.1038/nmeth.3099."
COMMENT     Sequence Label: pET28a-ybbR-HIS-sfGFP-DocI
FEATURES             Location/Qualifiers
     source          1..849
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             513..560
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
ORIGIN
        1 tgaaatttat ctgtaccacc ggcaaactgc cggttccgtg gccgaccctg gttaccaccc
       61 tgacctatgg tgttcagtgt tttagccgtt atccggatca tatgaaacgc cacgattttt
      121 tcaaaagcgc aatgccggaa ggttatgttc aagaacgtac catctccttt aaagacgacg
      181 gtaaatacaa aacccgtgcc gttgttaaat ttgaaggtga taccctggtg aatcgcattg
      241 aactgaaagg caccgatttt aaagaggatg gtaatatcct gggccacaaa ctggaatata
      301 atttcaatag ccacaacgtg tatatcaccg cagacaaaca gaaaaatggc atcaaagcca
      361 attttaccgt gcgccataat gttgaagatg gtagcgtgca gctggcagat cattatcagc
      421 agaatacccc gattggtgat ggtccggttc tgctgccgga taatcattat ctgagcaccc
      481 agaccgttct gagcaaagat ccgaatgaaa aacgtgatca tatggtgctg catgagtatg
      541 ttaatgcagc aggtattacc catggtatgg atgagctgta taagaaagtg gtacctggta
      601 ctccttctac taaattatac ggcgacgtca atgatgacgg aaaagttaac tcaactgacg
      661 ctgtagcatt gaagagatat gttttgagat caggtataag catcaacact gacaatgccg
      721 atttgaatga agacggcaga gttaattcaa ctgacttagg aattttgaag agatatattc
      781 tcaaagaaat agatacattg ccgtacaaga actaactcga gtaagatccg gctgctaaca
      841 aagcccgaa
//
