LOCUS       sequence-90747-e         957 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Probing molecule to perform single molecule force spectroscopy 
            experiments.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 957)
  AUTHORS   Otten M, Ott W, Jobst MA, Milles LF, Verdorfer T, Pippig DA, Nash 
            MA, Gaub HE
  TITLE     From genes to protein mechanics on a chip.
  JOURNAL   Nat Methods. 2014 Sep 7. doi: 10.1038/nmeth.3099.
  PUBMED    25194847
REFERENCE   2  (bases 1 to 957)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2014 Sep 7. doi: 10.1038/nmeth.3099."
COMMENT     Sequence Label: pET28a-ybbR-HIS-CBM-CohI
FEATURES             Location/Qualifiers
     source          1..957
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             4..26
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             76..99
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             106..123
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 aattttgttt aactttaaga aggagatata catatgggta ccgactctct ggaattcatc
       61 gcttctaaac tggctctgga agttctgttc cagggtccgc tgcagcacca ccaccaccac
      121 cacccatgga ctagtgctag catgtgcaat acagtatcag gcaatttgaa ggttgaattc
      181 tacaacagca atccttcaga tactactaac tcaatcaatc ctcagttcaa ggttactaat
      241 accggaagca gtgcaattga tttgtccaaa ctcacattga gatattatta tacagtagac
      301 ggacagaaag atcagacctt ctggtgtgac catgctgcaa taatcggcag taacggcagc
      361 tacaacggaa ttacttcaaa tgtaaaagga acatttgtaa aaatgagttc ctcaacaaat
      421 aacgcagaca cctaccttga aataagcttt acaggcggaa ctcttgaacc gggtgcacat
      481 gttcagatac aaggtagatt tgcaaagaat gactggagta actatacaca gtcaaatgac
      541 tactcattca agtctgcttc acagtttgtt gaatgggatc aggtaacagc atacttgaac
      601 ggtgttcttg tatggggtaa agaacccggt ggcagtgtag taccatcaac acagcctgta
      661 acaacaccac ctgcaacaac aaaaccacct gcaacaacaa taccgccgtc agatgatccg
      721 aatgcaggat ccgacggtgt ggtagtagaa attggcaaag ttacgggatc tgttggaact
      781 acagttgaaa tacctgtata tttcagagga gttccatcca aaggaatagc aaactgcgac
      841 tttgtgttca gatatgatcc gaatgtattg gaaattatag ggatagatcc cggagacata
      901 atagttgacc cgaatcctac caagagcttt gatactgcaa tatatcctga cagaaag
//
