LOCUS       sequence-90750-e         912 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Characteristic fingerprint molecule to perform single molecule force
            spectroscopy experiments; covalently immobilized via ybbr tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 912)
  AUTHORS   Otten M, Ott W, Jobst MA, Milles LF, Verdorfer T, Pippig DA, Nash 
            MA, Gaub HE
  TITLE     From genes to protein mechanics on a chip.
  JOURNAL   Nat Methods. 2014 Sep 7. doi: 10.1038/nmeth.3099.
  PUBMED    25194847
REFERENCE   2  (bases 1 to 912)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2014 Sep 7. doi: 10.1038/nmeth.3099."
COMMENT     Sequence Label: pET28a-ybbR-HIS-Xyl-DocI
FEATURES             Location/Qualifiers
     source          1..912
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             6..28
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             78..101
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             108..125
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ataattttgt ttaactttaa gaaggagata tacatatggg taccgactct ctggaattca
       61 tcgcttctaa actggctctg gaagttctgt tccagggtcc gctgcagcac caccaccacc
      121 accacccatg gactagtgct agcaagaatg cagattccta tgcgaaaaaa cctcacatca
      181 gcgcattgaa tgccccacaa ttggatcaac gctacaaaaa cgagttcacg attggtgcgg
      241 cagtagaacc ttatcaacta caaaatgaaa aagacgtaca aatgctaaag cgccacttca
      301 acagcattgt tgccgagaac gtaatgaaac cgatcagcat tcaacctgag gaaggaaaat
      361 tcaattttga acaagcggat cgaattgtga agttcgctaa ggcaaatggc atggatattc
      421 gcttccatac actcgtttgg cacagccaag tacctcaatg gttctttctt gacaaggaag
      481 gtaagccaat ggttaatgaa tgcgatccag tgaaacgtga acaaaataaa caactgctgt
      541 taaaacgact tgaaactcat attaaaacga tcgtcgagcg gtacaaagat gacattaagt
      601 actgggacgt tgtaaatgag gttgtggggg acgacggaaa actgcgcaac tctccatggt
      661 atcaaatcgc cggcatcgat tatattaaag tggcattcca agcagctaga aaatatggcg
      721 gagacaacat taagctttac atgaatgatt acaatacaga agtcgaaccg aagcgaaccg
      781 ctctttacaa tttagtcaaa caactgaaag aagagggtgt tccgatcgac ggcatcggcc
      841 atcaatccca catccaaatc ggctggcctt ctgaagcaga aatcgagaaa acgattaaca
      901 tgttcgccgc tc
//
