LOCUS       sequence-90753-e         826 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Characteristic fingerprint molecule to perform single molecule force
            spectroscopy experiments; covalently immobilized via ybbr tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Otten M, Ott W, Jobst MA, Milles LF, Verdorfer T, Pippig DA, Nash 
            MA, Gaub HE
  TITLE     From genes to protein mechanics on a chip.
  JOURNAL   Nat Methods. 2014 Sep 7. doi: 10.1038/nmeth.3099.
  PUBMED    25194847
REFERENCE   2  (bases 1 to 826)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2014 Sep 7. doi: 10.1038/nmeth.3099."
COMMENT     Sequence Label: pET28a-ybbR-HIS-10FNIII(x4)-DocI
FEATURES             Location/Qualifiers
     source          1..826
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             1..23
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             73..96
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             103..120
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tttgtttaac tttaagaagg agatatacat atgggtaccg actctctgga attcatcgct
       61 tctaaactgg ctctggaagt tctgttccag ggtccgctgc agcaccacca ccaccaccac
      121 ccatggacta gtgctagcgt tagtgatgtt ccgcgtgatc tggaagttgt tgcagcaacc
      181 ccgaccagcc tgctgattag ctgggatgca ccggcagtta ccgttcgtta ttatcgtatt
      241 acctatggtg aaaccggtgg taatagtccg gttcaagaat ttaccgttcc gggtagcaaa
      301 agcaccgcaa ccattagcgg tctgaaaccg ggtgttgatt acaccattac cgtttatgcc
      361 gttaccggtc gtggtgattc accggcaagc aacaaaccga ttagcattaa ctatcgtacc
      421 ggtagcggta gtggtagcgt ttcagatgtg cctcgcgacc tggaagtggt ggctgccaca
      481 ccgacctcac tgctgatctc atgggatgcc cctgccgtga ccgtgcgcta ttatcgcatc
      541 acatatggcg agacaggtgg caattcacct gtgcaagaat tcacagttcc tggttcaaaa
      601 agtaccgcca caatttctgg cctgaaacct ggcgtggatt acacaatcac agtgtatgca
      661 gtgacaggtc gcggtgatag tccggcaagt tcaaaaccga tttcaatcaa ttatcgcacc
      721 ggctctggct ctggctctgt tagtgatgtt ccgcgtgatc tggaagttgt tgcagcaacc
      781 ccgaccagcc tgctgattag ctgggatgca ccggcagtta ccgttc
//
